TECHNICAL CONFERENCES

Tuesday 22/05/2007 and Wednesday 23/05/2007 – 13:00 to 16:00
Room: Ogum

Luis Marcelo Bravo, Chile, Gerente de Negócios América Latina – Divisão Acadêmica / Invitrogen Corporation

Mini-curso Proteomics – Tornando mais fácil a análise de proteínas

Propomos, em formato de mini-curso, fazer um overview das etapas para estudo de proteínas com fornecimento de certificação. Abordaremos alternativas para preparação da amostra, separação e caracterização de proteínas e sua validação funcional.


Wednesday – 23/05/2007 – 10h30 to 12:00
Room: Ogun

Ana Paula de Andrade Aukar, Doutora em Genética e Melhoramento de Plantas, Assessora Científica da Eppendorf do Brasil

Estratégias para otimizar os resultados de PCR em Tempo Real e economizar reagentes.

A técnica de PCR em Tempo Real, ou PCR quantitativo está sendo muito utilizada atualmente na pesquisa científica e no diagnóstico para a quantificação de ácidos nucléicos, monitoramento da expressão gênica, detecção de patógenos, identificação gênica, taxa de SNP, genotipagem, ensaios +/- (Multiplexing), detecção End point, dentre outras aplicações. Vários fatores influenciam os resultados de um ensaio de PCR em Tempo Real como, por exemplo, a concentração inicial de ácido nucléico, concentração do primer usado, pipetagem, e outros fatores. Desta maneira, o preparo da reação de forma otimizada irá gerar dados confiáveis e ainda, trabalhando-se com sistemas eficientes de pipetagens é possível reduzir os volumes das reações e o número de replicatas, refletindo em economia para o laboratório.


Wednesday, 23/05/07 – 13:00 to 18:00h
Room: Chega Nego

Flávio Marques Peralta, Assessor Cientifico; LGC Biotecnologia, Brasil

Quimioluminescência 2007

O uso da quimioluminescência é hoje empregada em diferentes áreas da pesquisa, desde departamentos de bioquimica à proteômica, sendo utilizada para diversos propósitos A LGC Biotecnologia destaca o que há de mais novo em detecção quimioluminescente desde a detecção In Vivo ao uso de amplificadores de sinal quimioluminescentes.

Thursday, 24/05/07 – 10:30 to 12h30
Room: Chega Nego

Sandra Valéria de Sá, Ph.D.
Especialista de Aplicação da Roche Applied Science, São Paulo-SP, Brasil

GENOME SEQUENCER FLX: Flexibilidade em Diversas Aplicações.

O Genome Sequencer FLX é um sistema que oferece a solução completa para sequenciamento em alta escala, desde a construção da biblioteca até a análise dos dados. Plataforma que permite análise simultânea de vários genomas, gerando dezenas de milhares de bases em uma única corrida. Uma única pessoa pode sequenciar um genoma bacteriano em poucos dias, de forma economicamente eficaz. O Genome Sequencer FLX suporta inúmeras aplicações, desde a caracterização de DNA genômico até a identificação de transcritos e em estudos de regulação gênica.

GE Healthcare Life Sciences Lectures
Room: Omolu

Tuesday – 22/05/07 – 10:30 – 11:30h

Robert Marchmont
2D Label & Detection - Marketing Director, GE Healthcare Life Sciences, England

Advance Techniques in Proteomics
Advanced techniques in proteomics analysis are essential for detecting proteins, studying differencial expression and identify target proteins. At the same time, it has to be considered minimizing experimental errors, increasing reproductibility and sensitivity, optimizing results. Some of these techniques will be presented in this workshop.

Tuesday – 22/05/07 – 14:30 – 15:30h

Maurício Marques, PhD.
Field Applications Scientist, GE Healthcare Life Sciences, Brazil

Workshop Proteomics - DIGE
DIGE technology is a proven method that combines the most statistically accurate protein abundance data with the greatest resolving power available in 2-D electrophoresis. The presentation will include an overview, new products and applications of this technology.

Wednesday – 23/05/07 – 10:30 – 11:30h

Dan Schoeffner, Ph.D., Field Applications Scientist, Stratagene Inc.. USA

Quantitative PCR (Real Time PCR) Assay Design and Validation
A brief introduction to Quantitative PCR (QPCR) technology followed by a description of the benefits, methods and selection criteria for the design, validation and optimization of a new assay. Assay validation is necessary to determine the specificity of your primers and probes, the sensitivity, dynamic range, linearity and reproducibility of your assay. Additionally, this discussion will include methods of quantification and the appropriate use of normalizer/housekeeping genes.

Wednesday – 23/05/07 – 14:30 – 15:30
Hans Müller-Kahle, MBA
Industrial Business Development - Director
Biacore (part of GE)

Efficient Biotherapeutic Development Using Label-Free Protein Interaction Analysis
Presenting case studies, demonstrating the use of protein interaction analysis at key stages during development of biotherapeutics. Will focus on: * Antibody characterization for early selection of the most suitable candidates; * Detection and characterization of immune responses; The presentation will include examples of real, industry-based applications showing how protein interaction analysis using the latest Biacore systems can be used at every stage - from initial screening of crude hybridomas for lead selection through QC during manufacturing.

Wednesday – 23/05/07 – 16:30 – 18:00

Marcelo Anéas
Product Specialist, GE Healthcare Life Sciences, Brazil

Viviane Abreu Nunes Cerqueira Dantas
Instituto de Biociências – USP – Centro de Estudos do Genoma Humano, Brazil

Assessing the Regulation of T Cell Function by Distinct Cytokine Combinations on the Guava EasyCyte™ Plus Platform
Precise regulation of effector function is critical for mounting a potent, yet specific immune response to a given antigenic challenge. It has been hypothesized that the cytokine content of secondary lymphoid organs and at the actual site(s) of inflammation exert localized control over immune cell populations. Distinct cytokine combinations present within a given microenvironment can induce changes in the phenotype and effector functions of immune cells that differ dramatically from the effects exerted by each cytokine alone. Using a multiparametric analysis approach, we investigated the effects in culture of all possible combinations of as many as 6 cytokines (IL-2, IL-4, IL-7, IL-12, TNF-á, and TGF-ß) on the phenotype and functional capacity of CD4+ and CD8+ isolates derived from human peripheral blood. Following stimulation, cultures were assessed for changes in maturation/activation state (as measured by surface marker expression and phospho-protein analysis), as well as determining their individual proliferative capacity and viability (ViaCount®). Stimulated CD4+ and CD8+ cultures were also subject to cytokine production profiling through intracellular staining. The cytolytic capacity of CD8+ cultures was measured both indirectly, through intracellular Granzyme B staining, and directly through killing of autologous target B cells (CellToxicity™). All samples were acquired and analyzed using the Guava EasyCyte Plus platform, a microcapillarybased cell cytometry system. Briefly, we found that unique combinations of cytokines had profound and highly varied effects on lymphocyte fate and function in vitro. Demonstrations of synergy, antagonism, dominance, and substitution amongst multiple cytokines in our experimental system may reflect actual paradigms underlying such processes as the differentiation of T cell subsets into effector and resting memory phenotypes in vivo.


Thrusday – 24/05/07 – 10:30 – 11:30

David Willmot, Ph.D. Applications Scientist, Agilent Technologies, USA

Gene Expression, Comparative Genome Hybridization and other Applications of Agilent Microarrays in Genomic Research
Several high profile papers will be summarized in which Agilent in situ synthesized oligonucleotide microarrays surpass previous technologies. The Greenechip panmicrobial array custom designed for Columbia University is the first tool to provide comprehensive differential diagnosis of infectious diseases including viruses, bacteria, protozoa, etc. Agendia's MammaPrint breast cancer test is the first multi-gene expression test to receive regulatory clearance. The array identified a gene expression signature that reflects biological behavior of a tumor and out-performed all clinical variables for predicting patient survival. It provides classifiers to direct customized therapy. Agilent has developed a highly sensitive miRNA profiling assay, based on a highly efficient labeling method and novel microarray probe design. As little as 120 ng of total RNA is labeled and hybridized to an array containing all currently known microRNA sequences.

Thrusday – 24/05/07 – 15:30 – 16:30

Anna Heijbel, Senior Product Manager, GE Healthcare Life Sciences, Sweden

Maximize your purity and yield of histidine- or GST-tagged proteins
For simplification of protein purification and detection of your target protein histidine- and GST-tags are today the most widely used tags world wide. Let us show you our new product portfolio, get some hints and tips for solving your purifications problems with histidine- or GST-tagged proteins.How can you save time? How to minimize your sample preparation time?How to optimize your purification to increase purity?How and when to use the new tools for tagged protein purification With application examples and knowledge from our R&D labs we want to give you our perspective on how to purify histidine- or GST-tagged proteins in an optimized way.