Tuesday 22/05/2007 and Wednesday
23/05/2007 – 13:00 to 16:00
Room: Ogum
Luis Marcelo Bravo, Chile, Gerente de Negócios
América Latina – Divisão Acadêmica
/ Invitrogen Corporation
Mini-curso Proteomics – Tornando
mais fácil a análise de proteínas
Propomos, em formato de mini-curso, fazer
um overview das etapas para estudo de proteínas com
fornecimento de certificação. Abordaremos
alternativas para preparação da amostra, separação
e caracterização de proteínas e sua
validação funcional.
Wednesday – 23/05/2007 – 10h30 to 12:00
Room: Ogun
Ana Paula de Andrade Aukar, Doutora em
Genética e Melhoramento de Plantas, Assessora Científica
da Eppendorf do Brasil
Estratégias para otimizar os resultados
de PCR em Tempo Real e economizar reagentes.
A técnica de PCR em Tempo Real,
ou PCR quantitativo está sendo muito utilizada atualmente
na pesquisa científica e no diagnóstico para
a quantificação de ácidos nucléicos,
monitoramento da expressão gênica, detecção
de patógenos, identificação gênica,
taxa de SNP, genotipagem, ensaios +/- (Multiplexing), detecção
End point, dentre outras aplicações. Vários
fatores influenciam os resultados de um ensaio de PCR em
Tempo Real como, por exemplo, a concentração
inicial de ácido nucléico, concentração
do primer usado, pipetagem, e outros fatores. Desta maneira,
o preparo da reação de forma otimizada irá
gerar dados confiáveis e ainda, trabalhando-se com
sistemas eficientes de pipetagens é possível
reduzir os volumes das reações e o número
de replicatas, refletindo em economia para o laboratório.
Wednesday, 23/05/07 – 13:00 to 18:00h
Room: Chega Nego
Flávio Marques Peralta, Assessor
Cientifico; LGC Biotecnologia, Brasil
Quimioluminescência 2007
O uso da quimioluminescência é
hoje empregada em diferentes áreas da pesquisa, desde
departamentos de bioquimica à proteômica, sendo
utilizada para diversos propósitos A LGC Biotecnologia
destaca o que há de mais novo em detecção
quimioluminescente desde a detecção In Vivo
ao uso de amplificadores de sinal quimioluminescentes.
Thursday, 24/05/07 – 10:30
to 12h30
Room: Chega Nego
Sandra Valéria de Sá, Ph.D.
Especialista de Aplicação da Roche Applied
Science, São Paulo-SP, Brasil
GENOME SEQUENCER FLX: Flexibilidade em
Diversas Aplicações.
O Genome Sequencer FLX é um sistema
que oferece a solução completa para sequenciamento
em alta escala, desde a construção da biblioteca
até a análise dos dados. Plataforma que permite
análise simultânea de vários genomas,
gerando dezenas de milhares de bases em uma única
corrida. Uma única pessoa pode sequenciar um genoma
bacteriano em poucos dias, de forma economicamente eficaz.
O Genome Sequencer FLX suporta inúmeras aplicações,
desde a caracterização de DNA genômico
até a identificação de transcritos
e em estudos de regulação gênica.
GE Healthcare Life Sciences Lectures
Room: Omolu
Tuesday – 22/05/07 –
10:30 – 11:30h
Robert Marchmont
2D Label & Detection - Marketing Director, GE Healthcare
Life Sciences, England
Advance Techniques in Proteomics
Advanced techniques in proteomics analysis are essential
for detecting proteins, studying differencial expression
and identify target proteins. At the same time, it has to
be considered minimizing experimental errors, increasing
reproductibility and sensitivity, optimizing results. Some
of these techniques will be presented in this workshop.
Tuesday – 22/05/07 –
14:30 – 15:30h
Maurício Marques, PhD.
Field Applications Scientist, GE Healthcare Life Sciences,
Brazil
Workshop Proteomics - DIGE
DIGE technology is a proven method that combines the most
statistically accurate protein abundance data with the greatest
resolving power available in 2-D electrophoresis. The presentation
will include an overview, new products and applications
of this technology.
Wednesday – 23/05/07 –
10:30 – 11:30h
Dan Schoeffner, Ph.D., Field Applications
Scientist, Stratagene Inc.. USA
Quantitative PCR (Real Time PCR) Assay
Design and Validation
A brief introduction to Quantitative PCR (QPCR) technology
followed by a description of the benefits, methods and selection
criteria for the design, validation and optimization of
a new assay. Assay validation is necessary to determine
the specificity of your primers and probes, the sensitivity,
dynamic range, linearity and reproducibility of your assay.
Additionally, this discussion will include methods of quantification
and the appropriate use of normalizer/housekeeping genes.
Wednesday – 23/05/07 –
14:30 – 15:30
Hans Müller-Kahle, MBA
Industrial Business Development - Director
Biacore (part of GE)
Efficient Biotherapeutic Development Using
Label-Free Protein Interaction Analysis
Presenting case studies, demonstrating the use of protein
interaction analysis at key stages during development of
biotherapeutics. Will focus on: * Antibody characterization
for early selection of the most suitable candidates; * Detection
and characterization of immune responses; The presentation
will include examples of real, industry-based applications
showing how protein interaction analysis using the latest
Biacore systems can be used at every stage - from initial
screening of crude hybridomas for lead selection through
QC during manufacturing.
Wednesday – 23/05/07 –
16:30 – 18:00
Marcelo Anéas
Product Specialist, GE Healthcare Life Sciences, Brazil
Viviane Abreu Nunes Cerqueira Dantas
Instituto de Biociências – USP – Centro
de Estudos do Genoma Humano, Brazil
Assessing the Regulation of T Cell Function
by Distinct Cytokine Combinations on the Guava EasyCyte™
Plus Platform
Precise regulation of effector function is critical for
mounting a potent, yet specific immune response to a given
antigenic challenge. It has been hypothesized that the cytokine
content of secondary lymphoid organs and at the actual site(s)
of inflammation exert localized control over immune cell
populations. Distinct cytokine combinations present within
a given microenvironment can induce changes in the phenotype
and effector functions of immune cells that differ dramatically
from the effects exerted by each cytokine alone. Using a
multiparametric analysis approach, we investigated the effects
in culture of all possible combinations of as many as 6
cytokines (IL-2, IL-4, IL-7, IL-12, TNF-á, and TGF-ß)
on the phenotype and functional capacity of CD4+ and CD8+
isolates derived from human peripheral blood. Following
stimulation, cultures were assessed for changes in maturation/activation
state (as measured by surface marker expression and phospho-protein
analysis), as well as determining their individual proliferative
capacity and viability (ViaCount®). Stimulated CD4+
and CD8+ cultures were also subject to cytokine production
profiling through intracellular staining. The cytolytic
capacity of CD8+ cultures was measured both indirectly,
through intracellular Granzyme B staining, and directly
through killing of autologous target B cells (CellToxicity™).
All samples were acquired and analyzed using the Guava EasyCyte
Plus platform, a microcapillarybased cell cytometry system.
Briefly, we found that unique combinations of cytokines
had profound and highly varied effects on lymphocyte fate
and function in vitro. Demonstrations of synergy, antagonism,
dominance, and substitution amongst multiple cytokines in
our experimental system may reflect actual paradigms underlying
such processes as the differentiation of T cell subsets
into effector and resting memory phenotypes in vivo.
Thrusday – 24/05/07 – 10:30 – 11:30
David Willmot, Ph.D. Applications Scientist,
Agilent Technologies, USA
Gene Expression, Comparative Genome Hybridization
and other Applications of Agilent Microarrays in Genomic
Research
Several high profile papers will be summarized in which
Agilent in situ synthesized oligonucleotide microarrays
surpass previous technologies. The Greenechip panmicrobial
array custom designed for Columbia University is the first
tool to provide comprehensive differential diagnosis of
infectious diseases including viruses, bacteria, protozoa,
etc. Agendia's MammaPrint breast cancer test is the first
multi-gene expression test to receive regulatory clearance.
The array identified a gene expression signature that reflects
biological behavior of a tumor and out-performed all clinical
variables for predicting patient survival. It provides classifiers
to direct customized therapy. Agilent has developed a highly
sensitive miRNA profiling assay, based on a highly efficient
labeling method and novel microarray probe design. As little
as 120 ng of total RNA is labeled and hybridized to an array
containing all currently known microRNA sequences.
Thrusday – 24/05/07 –
15:30 – 16:30
Anna Heijbel, Senior Product Manager, GE
Healthcare Life Sciences, Sweden
Maximize your purity and yield of histidine-
or GST-tagged proteins
For simplification of protein purification and detection
of your target protein histidine- and GST-tags are today
the most widely used tags world wide. Let us show you our
new product portfolio, get some hints and tips for solving
your purifications problems with histidine- or GST-tagged
proteins.How can you save time? How to minimize your sample
preparation time?How to optimize your purification to increase
purity?How and when to use the new tools for tagged protein
purification With application examples and knowledge from
our R&D labs we want to give you our perspective on
how to purify histidine- or GST-tagged proteins in an optimized
way.