GE Healthcare Biosciences
The power of single-cell analysis & counting through microcytometry
Marcelo Anéas
Product Specialist
Guava Technologies has taken a fresh approach to “classic” applications. Guava has streamlined fluorescent cellbased assays and made them simpler by integrating instrument, software and reagents into a turnkey solution. When you upgrade to Guava cytometry solutions, not only do you gain many of the functional benefits of flow cytometry—you save so much in operating and acquisition costs. We call it the Power of Less.

DIALAB DIAGNÓSTICOS
PCR and RT-PCR: From Amplification to Cloning and Protein Expression – New
Methodologies.
Jayme Nunes de Souza Filho1; Ken Taylor2; Maria Carla Bastos Abdo3
1 Dialab Diagnósticos S/A; 2Integrated DNA Technologies-IDT; 3Promega Corporation
Recently, PCR-based techniques have been used to produce DNA fragments in order to be cloned. In routine, best primers associated with our MasterMix gives you easier and efficient way to amplify difficult templates. After adding dA at 3’ ends (Taq leaves it per si), those products may be cloned into linearized T/A cloning plasmid (i.e., pGEM-T), linked by
T4 DNA Ligase in just 1 hour. Also, reverse transcription reaction from mRNA needs to be taken in order to generate cDNAs to be cloned. Promega has offered an unique modified reverse transcriptase called ImProm-II which works up to 55 C without any Rnase activity; excellent for extension of long transcripts (up to around 9 kb of mRNA).
All those steps will be discussed including real-time PCR as quantitative assay using labelled primers (Plexor Systems) and new others methodologies such as transferring vectors (Flexi Vectors) and Interchangeable Labelling Technology (HaloTag).
UNDERSTAND YOUR NEEDS AND HELPING YOU ACHIEVE SUCCESS. IS A PRIORITY AT PROMEGA, IDT AND DIALAB.

GE Healthcare
Ettan MDLC – A new multidimensional liquid chromatography (MDLC) system for
proteomics applications.
Michael Murgu, PhD
Product Specialist
This new multidimensional liquid chromatography (MDLC) system for proteomics applications is the latest development in the range of LC systems from GE Healthcare. The Ettan MDLC provides dual functionality in terms of protein and peptide pre-fractionation and nanoscale multidimensional peptide separation of complex sample mixtures all in one
instrument. By providing on-column gradient monitoring, high throughput, wide dynamic range and a bio-inert flow path, this system helps to overcome most of the major challenges in today’s proteomics applications such as separation of labile peptides (e.g. post translational modifications) and analysis of low abundant proteins and biomarkers in complex sample mixtures.

GE Healthcare Biosciences
Comprehensive Analysis
Marcelo Anéas
Product Specialist
We show here a multi-disciplinary
The study was focused on ERK screening platform, for cell-based made with the DIGE technology CodeLink platform.

ABCV – Associação Brasileira de Ciências da Vida
O Custo dos Materiais importado no Brasil
Gisela Lis Bellinello
Diretora Executiva da ABCV
Sandra G. Reiser
Diretora Científica da ABCV

Invitrogen Brasil
Protoarrays: Nova ferramenta de análise de interações proteína -proteína
Marcelo Bravo
Invitrogen Brasil
O Microarray de proteínas é uma nova alternativa para identificar e caracterizar interações proteicas, permitindo a identificação rápida de novas interações entre milhares de proteínas em um simples experimento.

Invitrogen Brasil
Cytomics: Análise do Proteoma em Nível Celular
Carla Guimarães
Invitrogen Brasil
Utilização de anticorpos monoclonais como ferramenta de detecção de biomoléculas: visualização, quantificação e análise do produto da expressão gênica em nivel celular.

WATERS TECHNOLOGIES
Simultaneous Qualitative and Quantitative Protein Expression Analysis by LC/MS; Whole
Organism and Cell Proteomics and the Challenges of Complex, Dynamic Samples
Dr. LeRoy Martin
Waters-Micromass - UK
Current methodologies for protein quantitation include 2DE Gel Electrophoresis, stable isotope coded affinity tags (ICAT, GIST), metabolic labeling (15N), and stable isotope labeling with amino acids in cell culture (SILAC). Recently, there has also been great interest in using ion intensities of peptides derived from proteolytic digests for relative differential quantitation. While capable of many accurate quantitiative measurements, the many of the methods lack the ability to simply identify the peptides and thereby the proteins that are being monitored. In this work, we present methods based on accurate mass LC/MS where intact peptide ions are tracked for quantitative measurement, and co-eluting fragment ions are combined with these precursors for simultaneous qualitative assessment.

Nova Analítica
Proteomics Solutions at Thermo Electron Mass Spectrometry
Dr. Larry A. Burchfield
Latin America manager, Thermo Electron
Mass spectrometry in proteomics today addresses three broad application areas, identification of individual proteins from a mixture, quantification of proteins in a cell or organism and characterization of proteins to better explain its characteristics. Identification of all the proteins in a cell or organism is often a daunting task and poses a challenge even in the case of simple bacteria. Tandem mass spectrometry is currently used for protein identification studies. Ion trap mass spectrometers generate high quality data and represent the state-of-the-art in protein identification research.
Contents: Sample Prep - 2D Gel Electrophoresys and 2D LC/MS - Mass Spectrometry Analysis - Bioinformatics.

Eppendorf do Brasil
RealPlex – trazendo o futuro para o presente! Sistema Eppendorf para PCR em tempo
real
Luciana Gilbert Pessoa
Especialista de Produtos
Novo sistema para qPCR. Extremamente rápido, modular, flexível. Diversas opções ópticas para 2, 4 ou 6 filtros.
Especialmente projetado para facilitar qPCR, qRT-PCR, genotipagem, desenho do primer e sonda, seleção de marcadores fluorescentes, quantificação relativa e absoluta, medida de end point e análise de erros.

Eppendorf do Brasil
Como resolver problemas nas reações de PCR
Ana Paula Aukar
Especialista de Produtos
Soluções que vão desde diversos sistemas de extração de ácidos nucléicos até aplicação de enzimas especiais para por ex.: PCRs longos, complexos, de hot start, PCR em tempo real.

Eppendorf do Brasil
EpMotion 5070 e 5075: novas workstations Eppendorf automatizadas e semiautomatizadas
Carlos Eduardo Brancato
Especialista de Produtos Senior
Conheça as novas estações de pipetagem da Eppendorf. Sistemas automatizados que processam amostras a partir de diversos tubos ou placas e são indicados para melhorar a reprodutibilidade, produtividade e a precisão das técnicas utilizadas. Especialmente desenvolvidos para aplicações em biologia molecular.

Eppendorf do Brasil
Ferramentas para manipulação de células
Ana Paula Aukar
Especialista de Produtos
Apresentaremos sistemas que trazem soluções para Biologia Celular como injeções em diversos tipos celulares até a microdissecção de tecidos para posterior análise por diversas técnicas de biologia molecular. Sistema para produção de animais transgênicos. Soluções em eletrofisiologia: Patch clamp.

GE Heathcare Bio-Sciences
Pre-Clinical imaging
In vivo fluorescence imaging, physiology, anatomy and imaging function for research and
drug discovery
Sandra Rosa da Silva, Msc
Gerente de grupo de produto
Pre-clinical imaging have been utilizing by reserchers to visualize phenotypes, to study the progession of disease and to evaluate the efficacy of potential treatments in animal models. Pre-clinical imaging technologies such as small animal microCT, PET and and in vivo fluorescence optical imaging provide an avenue for more rapid and cost-effective
characterization of animal disease models. GE Healthcare pre-clinical imaging systems provide a comprehensive toolkit of pre-clinical sacanners and software applications for small animal anatomical and functional imaging.