A Putative Potato UDP-Glycosyl Transferase Involved In Defense Responses Against Biotic Stress
Agustín Correa1, Marcos Montesano1,2
1Laboratorio de Biología Molecular Vegetal, Facultad de Ciencias, Universidad de la República, Montevideo, Uruguay. Iguá 4225. 2Laboratorio de Biología Molecular, Instituto de Investigaciones Biológicas Clemente Estable, Montevideo, Uruguay. Av. Italia 3318.
Potato is the 4th crop produced in the world which is susceptible to a number of diseases such as soft rot. An etiological agent of these desease is Ewinia carotovora ssp carotovora causing important economical losses. Using mRNA differential display and further screening of a cDNA library, a complete cDNA, termed DRU13 (defense related UDP-glycosyltransferase), was isolated. The deduced amino acid sequence encoded by this gene contains typical UDP-glycosyl transferase domains. Northern blot analysis revealed a differential accumulation of the transcript, achieving maximum levels in plant tissues treated with E.c. carotovora-derived elicitors, whereas no detectable expression was observed in untreated plants or after injury treatment. Interestingly, DRU13 transcripts did not accumulate in response to treatment with several plant signal molecules known to be involved in defense responses, such as ethylene, salicylic acid and methyl jasmonate. This suggests that these molecules are not involved in the regulation of DRU13 gene in response to E.c. carotovora elicitors. The coding region of DRU13 was expressed in E. coli using the pQE-30 expression vector. The purified recombinant protein will be used for enzymatic assays and polyclonal antibody production. Furthermore, the sequence corresponding to the green flourescent protein (GFP) was fused to the N-terminal region of DRU13 to be able to perform localization studies in transgenic plants.
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