Docking studies of a Set of Inhibitors of Purine Nucleoside Phosphorylase from Schistosoma mansoni
Matheus Pereira Postigo1, Marcelo dos Santos Castilho2, Glaucius Oliva1, Richard Charles Garratt1, Adriano Defini Andricopulo1
1Laboratório de Química Medicinal e Computacional, Instituto de Física de São Carlos - USP; 2Faculdade de Farmácia, Departamento do Medicamento - UFBA
Tropical diseases are a major focus of special research
programs in the World Health Organization. One of such diseases is
Schistosomiasis, which affects about 300 million people around the World. Among
all species of Schistosomas, the only
found in Brazil is Schistosoma mansoni.
The lack of more effective and safe treatments for schistosomiasis has been stimulating
the search for new attractive biological targets as well as promising molecules
for drug research. Enzyme inhibitors play a crucial role in the pharmaceutical
industry. Parasite enzymes are attractive targets for drug discovery. The
enzyme purine nucleoside phosphorylase (PNP, EC 2.4.2.1) from Schistosoma mansoni is an important
target for the development of new chemotherapeutic agents. In presented work,
we describe the results of the development of structure-activity relationships
(SAR) for a set of inhibitors of S.
mansoni PNP. The family of 10 PNP
inhibitors examined is 9-substituted-9-deazaguanines. The bulk of the
structural diversity lies in the nature of the substituent linked to the
9-position of the purine ring. IC50 values were measured
employing inosine as substrate and a UV spectrophotometric assay, by varying
the inhibitor concentration at fixed substrate and enzyme concentrations. Molecular
modeling studies were carried out using the docking programs GOLD and FlexX. All docking simulations were carried out
with the X-ray coordinates of S. mansoni
PNP (available in our laboratories) to which hydrogen atoms were added in
standard geometry, after removal of the ligand and water molecules. SAR studies
revealed the essential interactions between the PNP active site and
small-molecule inhibitors. These studies have
identified the key residues in PNP catalytic mechanism, where the carboxamide
group of the Asn245 side chain forms a hydrogen bond with O6 of the purine
ring, while the side-chain carboxylate of Glu203 is close to accepting a
hydrogen bond from N1. According to the proposed binding model developed,
additional molecular modifications can be proposed for the synthesis of more
potent and selective inhibitors of PNP from S.
mansoni.
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