Obesity can be considered the major syndrome of 21st century. In obese individuals, alterations such as glucose intolerance, insulin resistance, dyslipidemia and atherosclerosis are frequently present increasing the mortality by cardiovascular diseases. Extracellular adenine nucleotides ATP, ADP, AMP and its nucleoside adenosine have several biological roles in the cardiovascular system. In the extracellular space these nucleotides can be hydrolyzed by soluble and cell surface located ectonucleotidases. Thus, it has been postulated that the activity of these enzymes is essential for the maintenance of ATP/ADP/AMP/adenosine appropriate levels contributing to vascular homeostasis. The consumption of a high palatable diet (cafeteria diet) is capable to induce metabolic alterations, being a decisive factor to increase fat mass formation as well as to developing of obesity-associated pathologies. The cafeteria diet consumption is positively associated with increase incidence of cardiovascular diseases. The aim of this study was to verify the influence of cafeteria diet on the ecto-nucleotidases activities in serum and its correlation with metabolic (glucose tolerance test, total cholesterol and triglycerides) and body variables (body weight and visceral fat mass). Twenty male Wistar of 60 days years old were separated in two groups: the control group (n=10) received standard laboratory rat chow and the diet group (n=10) received cafeteria diet for four months. All animals have free access to food and water. After perform glucose tolerance test animals were killed by decapitation, the blood was imediatelly collected and centrifuged at 2000 rpm by 10 minutes to obtain serum. Metabolic parameters were measured by commercial kits. The nucleotidases activity was determined by the inorganic phosphate release (malachite green method). The protein concentration was measured using Commassie method.The body weight, visceral fat mass, glucose tolerance test, total cholesterol and triglycerides in cafeteria group were statistically different from control group (p <0,05). The hydrolysis of ATP, ADP and AMP was respectively 32%, 50% and 23 % lower in treated animals compared to control group (p <0,05).In conclusion cafeteria diet can influence nucleotides hydrolysis, metabolic and body parameters increasing the risk of vascular and atherosclerotic diseases. Further studies are being performed to identify mechanisms and vascular lesions.Suportt: CNPq, FAPERGS, PROSPEQ/UFRGS