XXXV Reunião Anual da SBBqResumoID:9507


Proteolytic activity of honey from Tetragonisca angustula (Latreille, 1811; Apidae)
Oliveira, C. C.; Gomes, M.S.; Oliveira, F.; Coelho, M.V.

Instituto de Genética e Bioquímica. Universidade Federal de Uberlândia, Minas Gerais.

Many honeys of Meliponíneos have been used therapeutically, however without criterion. Studies of this material and its components could supply better, more necessary and more trustworthy indications on the medicinal use of these products. The purpose of the present work was to study the proteolytic activity on bovine fibrinogen of honey from Tetragonisca angustula, popularly known as jataí bee. The honey of jataí frequently is used by indians and besiegers for treatment of ocular infections. This study it was carried through with the honey of a strong colony, created in rational beehive PNN, urban area of Uberlândia-MG. Sample of honey was dialyzed against 0.05 M acetate of sodium, pH 4.0 buffer, for 24 hours at 4 ºC. After the dialysis, the honey was centrifuged (10.000 g) per 10 minutes and the precipitated was discarded.  The hydrolytic activity on bovine fibrinogen was evaluated by SDS-PAGE after incubating in (pH 4.0 – 10.0) buffer with 25 mL of dialyzed honey  and 25 mL of fibrinogen solution (3.0 mg/mL)ffer0 - 10.ferivity on bovine fibrinogen was avaluated by SDS-PAGE after icubating  in different times ( 15 – 90 minutes) at 37 ºC. The dialyzed honey cleaved the Aa chain faster than the Bb of bovine fibrinogen and showed no effect on g-chain. The Aa-chain completely disappears after 15 minutes of incubation. Bb-chain started to disappear at the same time but with a speed lower than that of Aa-chain, and partially disappear within 90 minutes. The proteases from honey are stable at high temperatures and presented maximum activity in pH about 6.0 and 8.0.The activity on the substrate fibrinogen indicates that the proteases are stable for 15 minutes at temperatures up to 60oC.  Higher temperatures affected substantially the enzymes. At the temperature of 70oC the enzyme partially lost its activity. Additional studies are necessary to verify if the therapeutical properties of the honey from T. angustula can be related to proteases, what it would give a great impulse for the studies of products of natural origin.