The oncogenic potential of Human Papillomaviruses can be mainly attributed to two oncoproteins, E6 and E7. The oncoprotein E7 (296bp) is the most powerful protein related with mutagenicity in cervical cancer. It interacts with retinoblastoma protein (pRB) deregulating the cell cycle. This process results in cervical lesions that can evolutes to cervical cancer. Considering this fact, E7 protein has been used as a potential tumoral specific antigen for vaccines development. The aim of this work was to clone E7 gene in Escherichia coli in order to produce and analyze the structure of this oncoprotein. The gene was amplified by PCR technique using specific primers in standard conditions. The reaction was performed in Rotor-Gene RG 3000 (Corbett Research) and purified with commercial kit. After that, PCR product was cloned into pGEM-T Easy Vector and transformed in DH5α strain of E.coli by heat-shock. The plasmid was extracted and sequenced in MegaBACE 750 (GE, Lifesciences). The raw data was basecalled using Cimarron v3.12 and submitted to BLASTN v2.2.13 to check the similarity between analyzed data and NCBI´s databank. PCR reaction resulted in unspecific fragments that were cleaned in the purification step. After transformation protocol, white colonies were isolated and the extracted plasmid was treated with restriction enzymes to release the insert. Among the plasmids submitted to sequencing, the sample with the highest score (94% on MegaBACE Scoreplate) matched with NCBI databank with score=587, E-value = 8e-165 and identity=100% (296/296). These results show that target sequence corresponds to HPV-16 E7 oncogene cloned into pGEM vector. This gene can be subcloned into yeast vector to be expressed with the purpose of prevent folding problems and hyperglycosilation in protein production.