Immunoenzimatic assays using biochemistry tests have been enough employed. In general way, these presents good diagnostic sensitivity, however complex technologies are involved and a long time of response is required, so new alternatives have been proposed. The SPR biosensor (Surface Plasmon Resonance) appear as promising alternative because it presents low cost, results in real time and simple operation. In SPR, while occur the formation of antigen-antibody complex, the thickness of the film on the altered sensory surface is detected by the displacement in the resonance angle providing in real time an electric signal susceptible of quantification. Like this to increase the diagnostic specificity in this sensors, was suggested a system of "SPR image", in order to reduce the quantity of positives false in the analysis when the measure are done in sensor surface with multiples zones of receptors active and inactive. This work, in contribution to SPR image development, had as major objective the study of methods of inactivation of receptors (antibodies) immobilized in solid surface that preserve actives the receptors of around micro regions. Plates of ELISA (NUNC – Immuno Plate Maxi Sorp) were used as model for immobilization of anti-Salmonella spp. and submitted to Ultraviolet (UV) light. Parameters like distance of UV source (5, 10 and 15 cm), time of exposition (15, 30 and 40 min) were evaluated . A medium inactivation of 99,98% was obtained when the plate was maintained to 5 cm of distance of the source for a great time of exposition of 45 min with antibody solution. These results suggest the use of the UV light on antibodies as capable to promote inactive zones permitting the production of SPR image sensors faster and selective.

Supported by: FINEP, CNPq, LIKA-UFPE