Interaction MBL2 and HBD1 Genes with Susceptibility to Human Papillomaviruses Infection. (HPV)
Brandão, L. A. C. 3; Guimarães, R. L. 3; Castelletti, C.H.M. 3; Simonetti, A.C 3; Souza, P. R. E. 3; Martins, D. B. G. 3; Crovella, S. 3,4; Arraes, L. C.2,4; Lima Filho, J. L. 1,3
1– Departamento de Bioquimica/UFPE; 2- Departamento de Medicina Tropical/UFPE; 3–Laboratorio de Imunopatologia Keizo Asami (LIKA/UFPE); 4- Dipartimento di Scienze della Riproduzione e dello Sviluppo. Sezione di Genetica, Universita di Trieste – Italy. lucabrand@gmail.com
The aim of this study is to investigate the association of three single nucleotide polymorphisms (SNP) in the mannose biding lectin-2 (MBL2) and one in the human b defensin-1 (HBD1) genes with susceptibility to human papillomaviruses (HPV) infection. MBL2 encodes a protein capable to activate the lectin pathway of complement system, stimulating an effective adaptive immune response as well as neutralizing the microorganisms directly. On the other hand, HBD1 encodes an antimicrobial peptide which disrupts the cytoplasmatic membrane of broad range of microorganism. Both proteins act as the first line of host's defense linking the innate with adaptive immune response. Previous studies have been described that HPV infection has a transitory pattern, where by most individuals eliminate the virus in 24 months. This pattern represent that host's immune response is involved in the progression and persistency of HPV infection. The study population included 40 HPV positive Brazilian patients and 165 healthy individuals, as control group. The analyses of the SNPs were carried out with real time PCR rotor gene 3000 (Uniscience, Corbett research) as platform using the melting and quantitative curve technology, for MBL2 and HBD1 respectively. The three SNPs in the exon 1 of MBL2 were assembled in the so called allele 0 when anyone mutation occurred. While the allelic promote region's mutation of HBD1 was called G. The results of frequency were analyzed by c2 and exact of Fisher tests. The 0 allele frequency in HPV positive (0,3) was different to the control group (0,2). The G allele frequency of infected group was 0,716 and 0,07 in control groups. The found results displayed that the presence of allele 0 and G influence the risk of HPV infection, increasing the susceptibility. Thus, they can be used in order to develop in new sorts of individuals treatments.
Financial support: LIKA/UFPE; IPIPE; CNPq
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