Cyclodextrin glycosyltransferase (CGTase, EC 2.4.1.19) is an enzyme capable of converting starch into cyclodextrin (CD) molecule. CDs are cyclic oligosaccharides compound of glucose units jointed by á-1, 4 linkages with a great potential to industrial usage. The most common types of cyclodextrins, á, -â- and ã-CD, consist of 6, 7 and 8 glucose units. CD is widely used in food, pharmaceutical, cosmetic, and agricultural industries. The most important characteristic of CDs is the ability of forming inclusion complexes with a great variety of compounds. The encapsulated compound has its physical and chemical properties changed to improve stability, volatility, solubility or bioavailability. In this study, the production of Bacillus sp subgroup alcalophilus E16 CGTase was made using cassava starch in submerged fermentation as substrate, and the crude enzyme was partially purified by ammonium sulfate precipitation at 80% saturation and gel-filtration chromatography. The aim of the partial purification of this crude enzyme is to apply it to CDs production in different kinds of carbon sources, issue that will be discussed in following researches.

Keywords: Cyclodextrin; Cassava starch; Cyclodextrin glycosyltransferase; Bacillus sp subgroup alcalophilus E16, Partial purification.