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Characterization Phylogenetic of Nocardiopsis sp of botecnological application isolated from soil of Northeast of Brazil
Furtado, V.C.3, 5; Viana, D. A.3; Souza, P. R.E. 3;Teixeira, M.F.S.4; Lima Filho, J. L.3,6; Araújo, J. M.1 and Porto, A. L. F.2, 3
1Departamento de Antibiótico, Universidade Federal de Pernambuco–UFPE; 2Departamento de Morfologia e Fisiologia Animal, Universidade Federal Rural de Pernambuco-UFRPE, 3Laboratório de Imunopatologia Keizo Asami, LIKA/UFPE; 4Universidade do Amazonas- UA; 5Universidade de Pernambuco, UPE; 6Departamento de Bioquímica-UFPE; Recife–PE, Brazil.
Actinomycetes are filamentous bacteria that naturally inhabit soils. They are of great importance in biotechnological processes because of their abilities to produce a large number of antibiotics and other bioactive secondary metabolites, such as enzymes. The aim of this work was to study phylogenetic characterization of strain Nocardiopsis sp. (IV) UFPEDA 3324 isolated from soil of Northeast of Brazil and antimicrobial activity detection. Study on the basis the phylogenetic analysis primer allelic specific sequence 16S rDNA (27f and 1525r) was carried out used amplified by the PCR and genus-specific pairs of primers Nsp2 (5´-TCTCTTGGGGTTGACGGTAG-3`) and Nsp1 (5´-TAAATGACCTCACATCTCT3´) for the selective amplification of the 16S rDNA regions of the genera Nocardiopsis. These microorganism was tested for antimicrobial activity against seven standard microrganisms, as gram positive and negative bacteria and yeast. The results obtained, showed antibacterial activity against S. aureus, B. subtilis and P. aeruginosa. Results of chemical characteristics of the peptidoglycan cell wall showed meso-diaminopimelic acid, and no are detected sugars (cell wall chemotype III/C). The phylogenetic analysis based on the results genus-specific of primers for a 16S rDNA sequence comparison revealed that the actinomycete represents the genus Nocardiopsis.
Financial support: FACEPE and CNPq.
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