Brazil has the greatest herd of bovine cattle (more than 200 million animals) and is the World´s largest exporter of beef, producing 7.6 million tons per year. The need to enhance genetic improvement in dairy and beef cattle has prompted research and development on bovine reproduction technology, which is crucial for sustainable growth of Brazilian agribusiness. The costs of imported FSH-related products, estimated to be around 2.5 million US$/year, greatly hampers further expansion of embryo transfer, a very useful technique for large-scale production of higher genetic standard cattle. In addition, all currently used porcine FSH preparations (such as Folltropin-V® or Pluset®) are not free of risk, due to the possible presence of the prion protein and, also, because they are not totally identical to the bovine preparation, giving rise to cross-species immunological reaction. Furthermore, their high content of LH hormone is considered to be responsable for the large variation in the superovulation results. The recombinant technology allows production of proteins of interest in a reproducible, practical and safe manner. The use of the heterologous mammalian cells system allows introduction of post-translational modifications, such as glycosylation, which has a major impact on FSH biological properties. Considering that, so far, no bovine recombinant FSH has been produced in Brazil, we set out to undertake this task. Here we show that the cDNAs corresponding to both α and β chains were amplified from RNA extracted from bovine pituitary. DNA sequencing was used to confirm successful amplification. Both cDNAs were cloned into the pCXN2 mammalian expression vector to be transfected into 293T cells. Recombinant bFSH is an essential tool for veterinary reproduction biotechnology.