Histopathological evaluation of excision cutaneous wounds topically treated using Parkia pendula seed lectin
Porto, C. S.1; Melo, C. M. L.1,2; Coriolano, M. C.3; Cavalcante, C. L. B.1; Lima-Filho, J. L.1,4; Correia, M. T. S.4; Coelho, L. C. B. B.4; Carneiro-Leão, A. M. A.1, 5
1Laboratório de Imunopatologia Keizo Asami LIKA/ UFPE; 2Programa de PG Ciências Biológicas, UFPE; 3Mestrado em Bioquímica, UFPE; 4Depto. Bioquímica, UFPE;5Depto. Morfologia e Fisiologia Animal, UFRPE
Lectins are proteins containing multivalent binding carbohydrates sites, which have a wide range of biotechnology applications. The aim of this work was to study the histopathological evolution of cutaneous wounds topically treated using Parkia pendula seed lectin (PpeL). Surgical round wounds (0.8 cm2) were produced aseptically in the dorsal region in albino Swiss mice (female, 8 weeks-old, 30.0 g, n=30/group). Each wound was evaluated and treated using daily doses, as follows: 150 mM NaCl (C Group) and 10 µg PpeL (T Group). Biopsies were purchased at 2nd, 7th and 12th days after surgery and wound samples were fixed in 4.0% (v/v) formaldehyde, processed to wax inclusion and stained using HE and Masson's Trichrome stainings. Analysis at the 2nd day showed similar aspects: highly vascular granulation tissue, polymorphonuclear infiltrate, neoangiogenesis and crust. At the 7th day, one could observe the presence of soft granulation tissue with fibrous characteristics and a rich-collagen extracellular matrix in the T Group. The C Group granulation tissue showed a vascular pattern (many blood vessels and poor collagen fibers). At 12th day, the Treated wounds were completely repithelized, showing a keratin epithelium and a great amount of organized collagen fibers. Besides this, the Control wounds showed partially repithelization, poor pattern of collagen fibers and still a large amount of miofibroblasts and crust. These results point experimental evidence to a potential use of PpeL as a biomaterial for therapeutic use.
Supported by: CNPq, FACEPE and CAPES.
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