Proteomic analysis of the serum may be marred if minor proteins are to be analyzed due to the presence of major components such as albumin and immunoglobulins G. These two classes of proteins represent about 50% of the total protein content of the serum. Alternative methods with potential to exclude these proteins from serum may prove to be of great value to serum proteomic analysis. The goal of this research was to produce, purify and immobilize IgG molecules against human serum albumin (HSA) and human IgG, which may be used as an affinity-binding matrix to exclude IgG and HSA, respectively. Anti-IgG and anti-HSA were produced by immunization of female rabbits with 200 µg of human IgG and HSA, respectively. Three boosts containing 400 µg of each protein were injected at periods of ten days after immunization. Aliquots of serum were obtained for the evaluation of the production of anti-IgG and anti-HSA. The sera containing the immunoglobulins were submitted to a partial purification by sodium sulfate and ionic exchange chromatography (DEAE-cellulose). SDS-PAGE was performed to evaluate the purification achieved. Immobilization of these proteins was carried out by crosslinking with glutaraldehyde using ferromagnetic Dacron as a matrix. After ten days of the third boost the titer of IgG was 1:5120 and 1:2560 for the serum containing anti-IgG and anti-HSA, respectively. The purification of these proteins by sodium sulfate precipitation and ionic exchange chromatography rendered a preparation presenting a high purification. Two major bands were observed in SDS-PAGE for both anti-IgG and anti-HSA preparations, representing the light and heavy chains of IgG. The immobilization of these proteins showed similar results regarding the amount of protein bound to the matrix: 330.6 µg of IgG per 50 mg of matrix. This represents 67% of the protein offerted to the matrix. The derivative IgG-ferromagnetic Dacron produced by the method describe herein can well be used as a biotechnological tool in affinity-binding methods as well as in immuno-diagnostic.