The anti-angiogenesis is a new therapeutic strategy that has being considered for the treatment of several pathologies like ophthalmic diseases, arthritis and tumors. The angiostatin is a potent anti-angiogenesis factor. It is an endogen protein derived from plasminogen and has four (4) regions denominated “kringles”. Smaller fragments of this protein maintain the anti-angiogenesis properties.  This work was developed with the objective to obtain a similar fragment to the three first kringles from the canine plasminogen and tests its anti-angiogenesis activity. The total RNA of the dog’s liver was extracted and used to obtain the cDNA. We have amplified the region correspondent to the three kringles. The product from RT-PCR was analyzed with agarose gel, demonstrating a band of approximately 740 bp. This DNA was inserted in vector pGEM-T, cloned in Escherichia coli and sequenced. The cDNA result demonstrated a central deletion of 62 bp compared with the sequence deposited in the GeneBank. The yeast P. pastoris (GS115) was electroporated with the pPIC9 vector sub-cloned with the cDNA. Analysis through the PCR showed the sequence insertion in the yeast genomic DNA. We utilized methanol to induct the protein production in shaker. The fermented supernatant was dialyzed, concentrated and analyzed through electrophoreses in gel SDS-PAGE and a band of 22 kDa was inducted by methanol addition. The recombinant protein was utilized to western blot and tests with bovine endothelia cells (C-PAE). The imunoblot demonstrated that the antibody polyclonal specific for human plasminogen do not recognize the recombinant protein, Tests with endothelial cells showed that, despite the deletion had altered the frame at the end of the sequence, 1,4 µg of the protein is capable to inhibit the cells growth in 80% when compared to the negative control. In conclusion the recombinant canin angiostatin fragment generated by the cDNA sequence having a possible alternative splice showed inhibition activity in the growth of the bovine endothelial cells “in vitro” what is a strong sign of anti-angiogenesis activity.