Plants seeds contain many proteins that might be involved in the protection of the dormancy seeds and the developing young seedlings against microbial infections. Among these proteins are chitinases, b-1,3-glucanase, antimicrobial peptides like thionins, defensins and lipid transfer proteins, all of which exert antifungal activity in vitro. The purification of antimicrobial peptides, including a LTP-like protein, from Phaseolus vulgaris (cv. perola) seeds were performed basically as described by Terras et al., 1992 with some modifications. Thirty grams of seed flours were extracted for 2 h (at 4 ^oC) with 150 mL of extraction buffer (10 mM Na₂HPO₄, 100 mM KCl, 2 mM EDTA, 1 mM phenylmethylsulfonylfluoride). The precipitades formed between 0 and 70% relative sulfate saturation were re-dissolved in distilled water and heated at 80 ^oC for 15 min. The resulting suspension was clarified by centrifugation and the supernatants extensively dialyzed against distilled water. A DEAE-Sepharose equilibrated with 20 mM Tris-HCl, pH 8.0, was initially utilized for the separation of peptides after ammonium sulphate fractionation. The basic fraction (the non retained D1 peak) represents the minoritary protein concentration. The electrophoretic analysis of D1 proteins obtained showed the presence of one unique band in SDS-tricine-gel with apparent molecular masses around 9 kDa. The immunoscreening of the fraction with an anti-LTP serum by Western blotting showed that the protein with molecular mass closer to 9 kDa cross-reacted with the antiserum indicating the presence of a LTP in the D1 basic fraction. Proteins of all samples were also visualized by SDS-Tricine-gel electrophoresis. The D1 fraction containing the LTP-like protein isolated also will be tested on a growth inhibition assay with the different fungi, such as Fusarium oxysporum, Fusarium solani, Colletotrichum lindemuthianum and Saccharomyces cerevisiae. The presence of LTPs in other important Brazilian seeds like Vigna vexillata, Canavalia ensiformis and Phaseolus vulgaris (cv. carioquinha) also was detected.