The cattle tick Boophilus microplus is an important ectoparasite that causes severe economic losses to livestock. In 2003 Seixas et al characterized a Vitellin Degrading Cysteine Endopeptidase from B. microplus eggs (VTDCE), which is involved on vitellin degradation during embryogenesis. This enzyme is a cathepsin L possessing maximal activity at pH 4.0. It is formed by two subunits, 17 and 22 KDa. Acidification of yolk granules is the main hypothesis to explain the vitellin degradation mechanism. Probably, this protease is also activated by acidification. Immunolocalization techniques showed the enzyme localized mainly in the ovary basal region and in vesicles located in the cellular pedicel region. As both correspond to material exchange areas, probably VTDCE has an extra ovary origin. Considering the possibility that VTDCE has an extra ovary origin the objective of this work is to investigate the VTDCE transport in hemolymph. A fluorogenic substrate (N-Cbz-Phe-Arg-MCA) was used to evaluate VTDCE activity. Inhibition assays were done using E-64, a specific inhibitor for cysteine proteases, and also with polyclonal antibodies against VTDCE. Partial purification of the enzyme from hemolymph and ovary extract was achieved using size-exclusion chromatography (Superdex 75) in a FPLC system. Finally, western blot technique was used to confirm the VTDCE presence in the samples. The activity present in hemolymph and ovaries extract was inhibited by E-64, confirming this activity comes from a cysteine protease. Inhibition by the specific antibodies confirmed that the enzyme present in the hemolymph and in the ovary is VTDCE. VTDCE activity elution profile in size-exclusion chromatography of hemolymph and ovaries extract were similar, suggesting the enzyme posses the same molecular weight in both tissues. In western blot, a high molecular weigh protein was recognized by antibodies against VTDCE, suggesting VTDCE could be associated with vitellin during its transport in hemolymph, and also in the ovary tissue. It would be  interesting to know the enzyme uptake mechanism and the involvement of a specific receptor.