Introduction: During the last years, the use of proteomics in toxinology has evolved considerably. Several reports have described the analyses of snake venoms using proteomic strategies. The Micrurus genus, so-called coral snakes (Serpentes, Elapidae), comprises 61 species distributed from the USA to South America. Venoms from these snakes are scarce and few is known about their protein content. From the toxinological viewpoint, these venoms diplay neurotoxic, hemolytic, myotoxic and cardiotoxic activities. Objective: Our goal was to analyse the protein composition of Micrurus frontalis and M. lemniscatus and to identify components from M. lemniscatus venom. Methods: The comparative proteomic analysis of M. frontalis and M. lemniscatus was accomplished by reversed phase chromatography and electrospray ionization mass determination (ESI-Q-TOF/MS). To assess the venom proteomic profiles from M. lemniscatus we used a combination strategy which inclued two-dimensional liquid chromatography, mass spectrometry and N-terminal sequence analyses. Results: The crude venom of the M. frontalis and M. lemniscatus showed large structural diversity when observed by ESI-Q-TOF/MS. However, M. leminiscatus venom diplays some molecular masses that are not observed in M. frontalis venom, such as molecular species at 4 and 8 kDa ranges. After N-terminal sequence determination, several proteins from M. lemniscatus showed strong similarity with others proteins from Elapidae venoms available at public protein databanks. The 6,678.02 Da protein showed 80% of the similarity with citotoxins and cardiotoxins from Naja naja venom. Neurotoxins from severals snakes display high similarity degree with 7,526.73 Da protein whereas 13,238.00 Da was shown to be similar to phospholipases. The higher protein observed in M. lemniscatus venom has a molecular mass of 22,532.74 Da and is probably an isoform of the kunitz inhibitors. Conclusion: The comparative proteomic analysis of these two venoms is a comprehensible catalogue of the secreted proteins. Among the totality of proteins from M. lemniscatus venom were identifided as neurotoxins, citotoxins, kunitiz inhibitors and phospholipases isoforms.