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Effect of the stem juice of banana, Musa paradisiaca.(Musaceae), on the cutaneous healing in mice
1Moreira, D.F.F; 3Mussi, S.V.; 4Lemos, F.O.; 3Ferreira, L.A.M.;4Lopes, M.T.P.; 1Pimenta, A.M.C.; 1,2 Borges,M.H.; 1De Lima, M.E.
1Laboratório de Venenos e Toxinas Animais–Departamento de Bioquímica e Imunologia–ICB/UFMG,Belo Horizonte/MG; 2Laboratório de Bioquímica e Química de Proteínas-FUNED,Belo Horizonte/MG; 3Laboratório de Tecnologia Farmacêutica–Departamento de Produtos Farmacêuticos–FAFAR/UFMG,Belo Horizonte/MG; 4Laboratório de Substâncias Antitumorais–Departamento de Farmacologia–ICB/UFMG,Belo Horizonte/MG.
The plant kingdom offers a rich source of many biologically active molecules and they are of great interest to the pharmaceutical, nourishing and cosmetics industries. These molecules represent a large group of compounds of diverse chemical origin and generally they are secondary metabolites, such as alkaloids, flavonoids and tannins. For a long time tannins were used for the treatment of diarrhea by oral administration and for the topical treatment of cutaneous hemorrhage. Furthermore, antitumoral and anti-microbial activities also are attributed to these compounds. Taninns such as gallic acid are found in the banana plant, which has been used in popular medicine as healing compound. Furthermore tannins diminishes the risks of infection or inflammation. In this work we investigated the influence of the juice of stem from Musa paradisiaca L., Musaceae (MsE) and one of its fractions in cutaneous healing on mice.The lyophylised extract of MsE (1.0%, 5.0% and 10.0%) and its fraction (0.1%) had been used to formulate a gel using as espessante the natrosol. Swiss mice were anesthetized, depilated in the back dorsum, where a superficial cutaneous injury was provoked using a sandpaper. After, the animals received topical application of daily doses (100 µg/mice) of the gel alone (control) or gel plus MsE extract or gel plus the MsE-fraction, during ten days or until the total healing of the injury. The diameter of injury was daily measured with pakimetry. Mice that received the gel with MsE incorporated showed a reduction time of healing, when compared with control group. Additionally, the injury was cured more rapidly when the MsE-fraction was incorporated into gel. Previous studies showed this fraction is rich in different tannins, which are known to binding to proteins. In conclusion the juice of stem from Musa paradisiaca and its isolated fraction diminishes the time of cutaneous healing in mice, validating somewhat medicine the popular use of tannins to this purpose. The improved of healing by MsE is not clear yet, but probably the interaction among tannins and proteins of skin are important in this process.
Support: CNPq and FAPEMIG.
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