A b-D-N-acetilglucosaminidase extracted and partially isolated from crustacean Artemia franciscana by ammonium sulfate precipitation and filtration gel chromatography on a Bio Gel A 1.5m was immobilized on ferromagnetic Dacron yielding an insoluble active derivative with 5.0 units/mg protein and 10.35% of the soluble enzyme activity. b-D-N-acetilglucosaminidase-ferromagnetic Dacron was easily removed from the reaction mixture by a magnetic field, was reused for ten times without loss in its activity. The ferromagnetic Dacron was better activated at pH 5.0 and 7.0. The immobilize enzyme presented Km of 2.91 ± 0.48 mM and optimum temperature of 50°C. Present the same thermal stable of the soluble enzyme and larger enzymatic activity at pH 5.5. b-D-N-acetilglucosaminidase-ferromagnetic Dacron showed better degradative capacity on heparam sulfate.