The yeast Saccharomyces cerevisiae presents a PKC MAP Kinase signal transduction pathway composed of Pkc1p, Bck1p, Mkk1p and Mkk2p, and Mpk1p. It was described that this cascade is involved in the control of the cell wall integrity, osmotic stress response, pseudohyphal growth and control of carbon metabolism. The pkc1 Δ mutant cells show a more drastic phenotype in response to the carbon sources than the other mutants of this pathway. This indicates that the Pkc1p may have other functions besides that related to the Map Kinase pathway. To investigate this possibility, we have performed a microarray based expression analysis to compare the global response to glucose and raffinose in a pkc1 Δ mutant and the correspondent wild-type strain W-303. We have used a commercial microarray slide composed of 5803 yeast ORFs plus controls, covering the role genome of S. cerevisiae. Our results shows that pkc1Δ  present a different gene expression pattern when compared with the wild type W-303 strain during the shifting from glucose to raffinose. Under derepression conditions, 99 genes present a different expression ratio in pkc1 Δ cells compared with the wild type cells. From those genes, we selected 28 that present an expression ratio at least two times less in pkc1 Δ cells when compared with wild type for further analyses by real time PCR.