Sugarcane (Saccharum spp) producing around 420 million t/year in Brazilian farmland and covering more than 20 million ha in others 90 countries worldwide, underscore the economic importance of this culture. In field conditions, under biotic and abiotic stresses like drought, the sugarcane has been significantly reduced in its productivity. Strongly related to the sugar-responsive gene expression, the drought tolerance researches has been drawn relevant linkages with induced changes in the normal steps of sugar metabolic pathways, involving a group of sugars such as trehalose, sucrose and fructose. ESTs research (Expressed Sequence Tags) may offer an efficient way to identify gene expression from mRNA. The present work aimed at identifying distinct expression of ESTs encoding enzymes for trehalose, sucrose and fructose in plants growing in different induced water stress conditions. Sugarcane variety SP80-3280 was led to grow under suppressed irrigation along 5,9,13 and 17 days. Using nylon filters arrays (macroarray) we analyzed the expression profile of sugarcane ESTs from SUCEST and hybridized with P³² cDNAs . The results were statistically analyzed by R Program and ESTs significantly (p < .05) were selected and submitted to public data bases. Using this approach it was possible to identify in differentially induced ESTs related to enzymes Trehalose synthase, Trehalose-6-phosphate phosphatase, Trehalase, Sucrose-synthase, Sucrose–6-phosphatase, Fructose-1,6-biphosphate aldolase and Fructose-biphosphate aldolase. Our results indicated that the ESTs were induced during all water stressed period. Analysing theses enzymes would provide important information about sugarcane growing under water stress.