Porcine circovirus (PCV), a member of the Circoviridae family, is a small, nonenveloped DNA virus containing a covalently closed circular single-stranded genomic DNA of about 1.76 Kb. Two genotypes of PCV have been recognized: PCV type 1(PCV1) was originally identified in the porcine kidney (PK15) cell line and considered to be nonpathogenic to pigs and PCV type 2 (PCV2) is now accepted as the major infectious agent involved in postweaning multisystemic wasting syndrome (PMWS), a new emerging swine disease worldwide since its occurrence in 1991. PCV contains three main ORFs, ORF1 encodes the viral replicase, ORF2 encodes the capsid protein, which represents the major structural protein of PCV and also the most variable and ORF3 encodes one protein involved in apoptosis. The aim of this work was to sequence different Brazilian PCV2 strains to determine the molecular variation and compare them with others different circovirus isolates. DNA was extracted from PCV2 infected tissues samples isolated from different towns of Espirito Santo and Minas Gerais. PCR was carried using primers to amplify the viral capsid sequence. The amplified fragments were cloned in pGEM T Easy vector then purified and sequenced. The obtained nucleotides sequences of Brazilians PCV2 ORF2 were compared to twenty-two circovirus sequences retrieved from GenBank. Alignment was done with multiple alignment program CLUSTALW 1.8 and sequence analyses were performed with MEGA vs.3.0 and an unrooted tree was constructed using the distance-based neighbor-joining method and 2000 bootstrap replicates. Sequence comparisons of ORF2 gene from Brazilian isolates shared 99.77% nucleotide sequence identity, when compared with PCV2 sequences from other countries showed 96.28% sequence identity and among all used sequences 70,94%. Two distinct groups were observed in the dendogram, one for poultry circovirus and other for porcine circovirus. In PCV2 cluster group the Brazilian PCV2 strains were closely related to PCV2 strains originating from China, France and England and distant from USA and Canada PCV2 sequences, demonstrating that probably, our virus was originated from Europe and Asia, and not from North America. Study of PCV2 genetic diversity and the existence of variants are important for a better understanding of these emerging diseases pathogenesis and for the ensuing development of prophylactic measures, once a link between capsid protein variation and PCV2 pathogenicity may exist.