XXXV Reunião Anual da SBBqResumoID:9077


STUDY OF CARBOHYDRATES PRESENT IN DIFFERENT SPECIES OF CLADODES FROM Opuntia ficus indica IN ACCORDANCE WITH THEIR AGE


Oliveira, E. M.1,2; Silva, N. H.1; Lima Filho, J. L.1,2; Brito, J. Z.2,3; Silva, M.P.C.1,2



1Departamento de Bioquímica, 2Laboratório de Imunopatologia Keizo Asami (LIKA), Universidade Federal de Pernambuco (UFPE), 3Centre de Tecnologia do Nordeste (CETENE), Recife-PE, Brasil


In the last years the palm (Opuntia fícus indica) has been used in some countries as vegetable or for consumption of its fruits in nature. This elapses of its nutrition value, that has the carbohydrates as the most abundant component, which find an wide field in the food industry application. The palm is of easy culture, low cost, found widely in the backwoods northeastern, supportting drought, used as cattle food, medicinal plant and cookery. The objective of the present study was to compare the content of carbohydrates present in four cladodes varieties of palm related to the age. Cladodes (racket) were  washed, dried, grinded and centrifuged at 10000xg for 10 min. Total sugars content was determined according to phenol/ sulfuric acid method, reducing sugars according to DNSA method and uronic acid  carbazol method. The results shown that the content of total sugars was higher in the Round palm (29,04mg/mL). Therefore, was observed that in all analyzed varieties, the tertiary cladodes presented  approximately the double of the carbohydrates then the quaternary cladodes. The analyzed samples were tertiary and quaternary rackets of the palms: Copena F1, giant, clone 20 and round, which presented the respective concentrations of sugars 20,16; 14,7; 21,57; 12,75; 27,96; 11,55; 29,04; 15,24 mg/mL. In the determination of reducing sugars in all the analyzed palm varieties, the hydrolysed crude extract presented higher concentration, however, the Copena F1 was the variety that showed higher value in both extracts, 2.4mg/mL in no hydrolysed and 3.7 mg/mL in the hydrolysed one. As regards to the uronic acid contents, the tertiary cladodes of the four varieties presented higher amounts then that the quaternary. The thin layer chromatography revealed the presence of the sugars galactose, xylose and arabinose, in the tertiary and quaternary cladodes of the four varieties of palm. In a general way, the tertiary cladodes showed to possesses a higher content of carbohydrates, indicating that the  eldest palms have a higher nutrition value and that this can vary in accordance to the type of palms.

 

Suporte Financeiro: FACEPE, CAPES, CETENE e UFPE