Paw Edema and Pleurisy Induced-Carrageenan: Action of Major Types of Commercials Carrageenans
Silva, F. R. F.; Dore, C. M. P. G., Souza, R. L. A. ; Souza, M. C. R.; Queiroz, K. C. S.; Alves, L. G.; Medeiros, V. P.; Azevedo, T. C. G.; Dantas, J. C. M.; Rocha, H. A. O.; Chavante, S. F.; Leite, E. L..
1Departamento de Bioquímica, Universidade Federal do Rio Grande do Norte.
*Corresponding author. E-mail:eddaleite@cb.ufrn.br ,fax: 84 211-9208, Departamento de Bioquímica, Centro de Biociências, Campus universitário, Lagoa Nova,s/n, CEP 59082-970, Natal-RN, Brasil
The iota, kappa and lambda commercial carrageenans are polysaccharides from red seaweeds extensively used in food, cosmetic and pharmaceutical industry. These compounds consisted mainly of units of sulfated galactose and anhydrogalactose. Paw edema was induced by injecting carrageenans (kappa, iota and lamda) in saline into the hind paw of a male Wistar rats. Injections of carrageenans into the pleural cavity of rat were also used in the induced an acute inflammatory response characterized by fluid accumulation in the pleural cavity. All three types of carrageenans showed a large number of neutrophils and raised NO production in pleural exudates. The kappa and lambda- carrageenans showed respectively a volume of exudate of 1.28 ± 0.05 and 1.35 ± 0.02 and of nitrite/nitrate of 25.04 ± 0.71 and 33.17 ± 0.88 nmol to nitrite/nitrate. The iota -carrageenan caused the greater inflammation in relation to the other and showed high concentration of nitrite/nitrate (63.478 ± 2.58 nmoles/rat), exudates volume (1.52 ml) and PMNs (4902 x 103 cells). The histological analysis demonstrated that kappa and iota carrageenans (1%) have a major cellular infiltration. Thus, was demonstrated in quantitative evaluation of inflammations that the ι-carrageenan have major inflammatory potential in relation the others carrageenans.
Supported by CAPES and CNPq.
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