Erythropoietin (EPO) is a hormone that belongs to a group of growth hematopoeitics factors, which control the proliferation and differentiation of marrow bone´s cells. It acts inducing the elevation of the production of blood red cells, increasing the amount of circulating hemoglobin and oxygen. This hormone, mostly secreted by kidneys, is widely used in medicine as a treatment for anaemia, kidney and heart disorders, tumors and numerous diseases. Recombinant EPO has been produced in COS-1 and CHO cells and has been experimentally inserted in insect, yeast and bacterial cells. Up to now, to the best of our knowledge, there is only one description of transgenic expression of this protein in plants. In the present work we intend to evaluate the expression of human EPO in plants such as rice (Oryza sativa) and tobacco (Nicotiana tabaccum). The EPO gene was synthesized based on the nucleotide “overlapping” technique. A fragment of 582 bp was cloned into pCR-Blunt vector (Invitrogen) and then submitted to automatic sequencing. At that time, a mutation on nucleotide 252 (G→A) was identified. It did not modify the encoded amino acid of the codon, a glycine. Therefore, once the identity of the sequence was confirmed, this fragment was transferred to the expression vector pWUbi.tm1, armed with the promoter of the ubiquitin gene and the terminator tm1’. This expression cassette was transferred to the binary vector pWBVec4a, which was then transformed into strains ALG1 and LB4404 of Agrobacterium tumefaciens. These strains will be employed in the transformation of rice and tobacco, respectively. Transgene integration into plant genomes will be evaluated by PCR and Southern blot hybridization. In order to confirm the expression of EPO in plants, we will be performing Northern blot and Real-Time PCR analysis. The presence of the protein will be evaluated by SDS-PAGE and immunodetection.