Angiogenesis, the sprouting of new vessels from pre-existing vessels, impacts on many important pathological states including cancer, ischemic cardiovascular disease, wound healing and inflammation. Such process is highly dependent on the proliferative activity of vascular cells. A novel benzopyran derivative compound was identified in Curvularia sp., an endophytic fungus associated with Ocotea corymbosa (Lauraceae). Such compound was purified by chromatographic methods and the preliminary analyses of its biological activities showed that it was able to induce proliferation to mammalian cells. Based on such results, we aimed to investigate the angiogenic activity of the benzopyran compound using an "in vivo" model, the chorioallantoic membranes (CAM) assay using eggs from Galus domesticus. After exposure to the compound, the CAM were analyzed histologicaly and by different softwares aiming at to quantify the number of vessels. The quantitative analysis on the CAM revealed a 70% increase of blood vessels density in comparison with the controls (n=9). The histological analyses also evidenced an increase in the number of blood vessels and fibroblasts present in the treated CAM. Hence, it is possible to conclude that this novel benzopyran is able to induce angiogenesis and fibroplasy, prompting us to further characterize such activities and possible applications.