Some cultivars of bell pepper are resistant against some plant-pathogens of important commercial cultures. Leaf Antimicrobial Peptides (AMPs) could account for this defense system, as an innate defense. Previous results in our laboratory indicated that two partially purified extracts, Soluble Extract (SE) and Cell-Wall Extract (CWE), obtained from bell pepper leaves ('Sunshine' var.), inhibited total or partially the growth of two plant-pathogens. This work aims to improve the purification steps of these bell pepper extracts, in order to obtain high peptide amounts for antimicrobial and structural characterizations, including identification of interest peptide forms by means of mass spectrometry (MS). Leaves from bell pepper plants were powdered, macerated with Tris-HCl added of protease inhibitors and centrifuged. The supernatant was saved, the precipitate was washed twice (water), and the resulting supernatants combined with the saved one, named Raw Soluble Extract (RSE). The obtained precipitate was extracted with LiCl added of EDTA and proteases inhibitors. The mixture was stirred, centrifuged and the supernatant was referred to as Raw Cell-Wall Extract (RCWE). RSE and RCWE were separately fractionated (ammonium sulfate and heating) and centrifuged, originating SE and CWE, respectively. SE was separated in a Sephadex G-10 column. SDS-Tricine-PAGE indicated a peptide-enrichment in two areas of the eluted profile. At the first area, we suggest that interactions among peptide has occurred, producing agglomerated structures. These two regions were mixed and SE was separated in a Sephadex G-50 column. Preliminary MS analyses indicated the presence of peptides lower than 6 kDa. So, these samples were alquilated and submitted to partial proteolyses, aiming the obtainment of the sequences and the identification of the peptides. Considering that unsatisfactory results were obtained, since we have complex samples, the recovered polls are being  separated in a C18-RP-HPLC. CWE was separated in a Phenyl Sepharose column and some pools were recovered and are being separated by C18-RP-HPLC. Isolated recovered pools are being again analyzed by MS. The identification of AMPs by MS, followed by other characterizations and by the expression of these molecules could permit the determination of the 3D structure and the identification of the potential use of these molecules for human benefits. Supported by FAPEMIG, CNPq and RENABIME-LNLS.