The mycelium analyzed in the present work was obtained from the ascomycete Botryosphaeria rhodina grown on glucose as sole carbon source. This fungal isolate produces an exopolysaccharide (EPS) of the b-1,3;1,6-D-glucan type named botryosphaeran. The mycelium was separated from the fermentation broth, which contained the EPS by centrifugation (9000 rpm/20 min/4 ºC), and then exhaustively washed with distilled water until the washings showed no sugar detectable by the phenol-sulfuric acid method. The mycelium, free of EPS, was then subjected to three different extractions treatments: cold water (5 °C, 3 x), hot water (100 °C, 4 x), and alkaline solution (2 % w/v, KOH, 1 x) treated before with NaBH₄. The extracts obtained from each extraction procedure were separated from their respective residues by filtration followed by centrifugation. Next, they were reduced to a small volume on a rotary evaporator. To this were added 4 volumes of absolute ethanol, the precipitates collected by centrifugation and re-dissolved in distilled water and dialyzed. Aliquots of these fractions were withdrawn for the determination of protein and total sugars. Ethanolic precipitates from the cold-water extractions contained high total sugar and protein, while the hot-water (PEEaq) and the aqueous-alkaline extractions (PEEAlc) showed the highest total sugar concentration with a low proportion of protein. Aliquots of the ethanolic precipitates arising from all 3 types of extraction were hydrolyzed with 5.0 M TFA/16 h/100 ºC (Gutiérrez et al., 1996A), and analyzed by HPAEC. Hydrolysates from the cold and hot water extractions showed mainly glucose, while hydrolysates from the alkaline-extracted mycelium also contained fucose, galactose and mannose, as well as glucosamine. FT-IR spectra of the PEEAq fraction showed one band at 890 cm^-1, characteristic of a glucan in the b-configuration, and PEEAlc spectra also showed one band at 810 cm^-1, indicating the probable existence of a polysaccharide of the a-configuration type (Gutiérrez et al., 1996B, Schweiger-Hufnagel et al., 2000, Zhao et al., 2005).