Major depression is the most common psychiatry disorder and learned helplessness paradigm is the most widely accepted animal model to studies this disease. Glutamate is a neurotransmitter involved in several physiologic functions as learning and memory, besides being involved in some psychiatric disorders, as schizophrenia and own depression. The objective of our work was to investigate the glutamate uptake in hippocampus, cortex and striatum slices of animals submitted to the learned helplessness paradigm. Female rats were used, and submitted to inescapable shocks and 24 hours after, to escapable shocks. The glutamate uptake assays were performed immediately, 24h and 21 days after the last shocks session. The animals with cognitive deficit were classified as Learned Helplessness. The results showed alteration in the glutamate uptake in slices of hippocampus between the Learned Helplessness animals and the not Learned Helplessness. Our results corroborate with the recently idea that alterations of the glutamatergic system are involved with the depression behaviour and this system can be a potential target of the antidepressant drugs.