There are in literature several examples of use of synthetic and natural polysaccharide as support for enzyme immobilization due the versatility of chemical reactions these materials offer, the biodegradability and biocompatibility. One of the limitations in enzyme immobilization is the formation of two phases, with enzyme held in solid phase, resulting in restrictions of mass transfer and diffusion limitation. The use of soluble polysaccharides as support represents a potential solution for this problem. In this work Horseradish peroxidase (HRP) was immobilized in Anacardium occidentale L. polysaccharide (CGP). Immobilization was made adding 1000µL of HRP (24 U mL^-1) to 15 mg periodate activated CGP and then, left mixture to react for 30 min at 4ºC. After that, CGP-HRP was precipitated by ethanol or alternatively by isopropanol, acetonitrile, acetone, ethylic ether, chloroform, hexane and dimethyl sulfoxide (DMSO). The activity was measured after 1min reaction using pyrogallol (0.07mol L^-1) and H₂O₂ (0.05mol L^-1), pH 6.0. The value obtained in the blank reaction, performed in the presence of activated CGP, was discounted from all the readings. The reusability of the CGP-HRP was tested in all solvents, and in then case of acetone in presence of 1.0 mol L^-1 of sodium phosphate. All tests were performed in triplicate and results presented as average plus standard deviation. The CGP-HRP presented activity after precipitation with all tested solvents. The best performance was obtained when acetone was used to precipitate CGP-HRP, with 100% of activity in the second use. In decreasing order, the treatment with solvents resulted in activity maintenance of 93.3% for propanol, 42.4% for DMSO, 20.2% for ether, 17.6% for ethanol, 9.6% for acetonitrile, 8.7% for hexane and 1.3% for chloroform. The best result of reusability of CGP-HRP was obtained when acetone and sodium phosphate were used to precipitate the immobilized enzyme. In this condition the CGP-HRP was used three times with 100% activity, with a decrease of 37% of activity after fourth use.  The results of this work are very promising, and indicate CGP as a very good support to perform biocatalysis with HRP on non-conventional medium, organic or high salt concentration medium.