The phosphorus acquisition system in Neurospora crassa includes four regulatory genes: nuc-2, preg, pgov and nuc-1. When the mould is grown on high Pi, the PREG/PGOV complex regulates negatively the transcription factor NUC-1, which is responsible for the expression of the Pi-repressible phosphatases, phosphate permeases and nucleases. When grown on limiting Pi, the PREG/ PGOV complex is regulated negatively by NUC-2, which senses the availability of Pi in the cell. The NUC-1, PREG, PGOV and NUC-2 have similarity respectively to PHO4, PHO80 PHO85 and PHO81 from Saccharomyces cerevisiae. PHO80 and PHO85 form a cyclin-CDK complex, which phosphorylates PHO4. Nevertheless, the role of preg gene in N. crassa still remains controversial, i.e., the preg^c mutant (loss of function mutation) secretes on high Pi medium different molecular forms of the acid phosphatase, as compared to those secreted by the wild-type strain grown on limiting Pi. Thus, PREG might also be involved in regulating posttranslational modifications of the Pi-repressible phosphatases. Here, we identified novel genes controlled by PREG using DDRT-PCR.  Total RNA was isolated from the 74A and preg^c strains grown on low-Pi medium adjusted to pH 5.4 and 7.8. The differentially expressed bands were purified and cloned into the plasmid pGEM-T. After sequencing and BLASTx analyses of the reads, the following differentially expressed transcripts were identified: an uracil-5-carboxylate decarboxylase, a metiltransferases ubiE/COQ5 and hypothetical proteins showing similarity to putative motifs for b-lactamases and dehydrogenases dependent of FMN (FMN_dh). The low expression of the uracil-5-carboxylate decarboxylase transcript was confirmed in the preg^c mutant by Northern blotting. Therefore, our data suggest that, in addition to its role in the acquisition of Pi, preg gene might regulate other cellular processes, as the transcription of an uracil-5-carboxylate decarboxylase involved in the thymidine salvage pathway.