Antimicrobial peptides (AMPs) constitute one of the first barriers to pathogenic invasion, and could be constituent or induced in organisms as plants and animals. The objective of this work was to extract and characterize AMPs from bell pepper of the commercial variety Magali R. Fully-expanded leaves were powdered (liquid N₂), macerated with Tris-HCl buffer, EDTA, PMSF and thioureia, the homogenate was lyophilized, centrifuged and the recovered supernatant was named Soluble Extract (SE). The precipitate was washed (water, 3x), extracted with LiCl, thioureia, benzamidine and PMSF, the homogenate was centrifuged and the recovered supernatant corresponded to the Cell-Wall Extract (CWE). SE and CWE were separately fractionated with ammonium sulfate (35-65% sat.), following gel filtration (Sephadex-G50, Pharmacia, 200 mL, f=2.0 cm). The elution profile of gel filtration obtained for SE separation presented two peaks, while for CWE yielded a single peak. SDS-Tricine-PAGE was used to localize enriched-peptide fractions. The results indicated that the whole single peak obtained from CWE is rich in peptides, while just the second obtained peak of SE presented peptides. Enriched-peptide fractions were submitted to hydrophobic interaction chromatography (Phenyl Sepharose - Pharmacia, 150 mL, f=2.0 cm). The CWE separation yielded eight promising hydrophobic peaks (HPs), and in SE separation, nine HPs were selected. Only the HPs of CWE were evaluated by the inhibition test against the plant-pathogenic bacteria Ralstonia solanacearum and Clavibacter michiganensis subsp. michiganensis. The largest inhibition rates were obtained with the HP3 (50.9% for the first bacterium and 57.3% for the other one) and HP4 (62.3% for the first bacterium and 74.0% for the other one). After gel filtration, the detection of peptides in whole CWE single peak, if compared with the location only in the SE second peak, suggests a preferential location of AMPs in the periphery of the cell, according to the function of primary defense.  The high inhibition rates of the plant-pathogens growth by the partially purified fractions of CWE can also be observed. Those fractions will be purified by C4 ou C18-RP-HPLC and peptides will be sequenced by MS/MS. The generated results will make possible to develop the AMPs cDNAs cloning in an heterologous system. In the future, the identified AMPs could be used in agribusiness or as antimicrobial agents for animals.  Supported by FAPEMIG and RENABIME-LNLS.