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2D Electrophoresis Reveal Xanthomonas
axonopodis pv. citri Proteins Expressed on Synthetic Non-Induction and
Induction medium for virulence and pathogenicity factors
Ferreira, R M.1;
Facincani, A.P.1; Soares, M.R.2; Gozzo, F.C.2;
Ferro, M.I.T.1; Ferro, J.A.1; Meneghini, R.2; Pizauro Jr, J. M.1 and Oliveira,
J.C.F.1
1Department of Technology, FCAV-UNESP-Jaboticabal-SP-Brazil; 2Brazilian Synchrotron Light Laboratory, CNPq-Campinas-Brazil.
Citrus canker is a plant disease caused by
the gram-negative phytopathogenic bacteria Xanthomonas axonopodis pv citri
(Xac). Such disease is considered one of the most destructive to citrus
crop, as in the absence of chemical or genetic control methods, eradication
become the lonely efficient method to fight against the pathogen. Disease
symptoms are characterized by localized host cell hyperplasia followed by
tissue necrosis on leaves, stems and fruits of citrus host plants. At the Xac
post-genomic stage, we have been undertaking a 2D-electrophoresis Proteomic
approach to analyze Xac protein expression on synthetic culture mediums.
The two-dimensional electrophoresis profiles for Xac growing during 24
hours in Nutrient and in XVMB medium were compared. Total protein extracts from
both conditions were analyzed by 2D-gels and differentially expressed protein
spots were digested with trypsin and the resulting peptides were analyzed on a
MALDI TOF-TOF mass spectrometer. The ImageMaster Elite software (Amersham
Pharmacia) identified about 600 spots for each condition and 150 of them have
shown to be differentially expressed when nutrient medium condition is taken as
reference, as follow: 46 down-regulated or completely knocked-off proteins, and
104 up-regulated or expressed De Novo proteins. A panel of
pathogenicity, virulence and adaptation proteins was shown to be induced when Xac
was grown in XVMB, demonstrating that XVMB can mimics plant-contact response at
early stages of infection.
Financial
Support: Auxílio Jovem Pesquisador FAPESP and FUNDECITRUS
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