Myeloperoxidase inhibition by extracts from Maytenus ilicifolia (Piperaceae)
Barbosa, VF; Vellosa, JCR; Khalil, NM; Brunetti, IL; Furlan, M; Oliveira, OMMF
Departamento de Bioquímica e Tecnologia Química, Instituto de Química, UNESP-Araraquara (SP)
Introduction: The myeloperoxidase (MPO) is an enzyme from neutrophils that participates in oxidative process in different pathological conditions (Atherosclerosis, Arthritis, Alzheimer disease).
Objective: Many natural products are used in diseases treatment. The aim of this study is evaluate the M. ilicifolia (Piperaceae) potential as a source of MPO inhibitors. There were evaluated: crude ethanolic extract (CEE) and its fractions, hydroalcoholic (Hid), hexanic (Hex), and hexane-ethylacetate (HexAcOEt)
Methods: Assays (Phosphate buffer 50mM, pH7,0; Vf=1mL; 250C) were followed with, or without extracts in different concentrations, guaiacol 20mM, MPO 100mU and H2O2 0,2mM; Inhibitions were compared by IC50 values. The absorbance raise was read in 470nm during 1 minute after initiate the reaction with hydrogen peroxide.
Results: It was compared media+DP, considering p<0,05; IC50 values: CMICRE (0,0040mg/mL), Hid (0,0022mg/mL), Hex (was not able to inhibit in 50%) and HexAcOEt (0,0042mg/mL).
Conclusions: In this work, M. ilcicifolia extracts interposed in the guaiacol oxidation by MPO/H2O2. As we can see, by the presented results, the inhibition power follows, from the best to the worst: Hid, CEE, HexAcOEt and Hex.
e-mail: josevellosa@yahoo.com.br
Supported by: FAPESP, BIOTA and CAPES
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