XXXV Reunião Anual da SBBqResumoID:8377


Glucose Metabolism During Aedes aegypti Embryo Development

Vital, W. O.1; Moraes, J.1; Rezende, G. L.2; Lemos, F. J. A.1and Logullo, C1.



1) Laboratório de Química e Função de Proteínas e Peptídeos and Laboratório de Biotecnologia - CBB - UENF, Campos dos Goytacazes, RJ, Brazil; 2) Laboratório de Fisiologia e Controle de Artrópodes Vetores, Departamento de Entomologia – FIOCRUZ, Rio de Janeiro, RJ, Brazil.


In the present study we investigated glucose metabolism during the embryogenesis of the mosquito Aedes aegypti. An increase in glucose and glycogen content during the A. aegypti embryonic development was detected and showed to be due to the high enzyme activity of gluconeogenesis and concomitantly low glycolisis activity. Glucose 6-phosphate (G-6P), formed by hexokinase, enzyme that catalyze the first step of glycolisis, at the initial part of embryogenesis is driven mainly to pentose phosphate pathway, in order to produce fundamental substrates for cellular biosynthesis. However, after cellularization (10h) it is driven mainly to glycolytic pathways as energetic substrate to embryo development. This fact was confirmed by the detection of an increase in hexoquinase and pyruvate kinase activities after the embryo cellularization. Accumulation of key metabolites such as glycogen and glucose was monitored and revealed that their concentration increases from hour 0 up to hour 15 after oviposition. We detected the Glycogen Synthase Kinase (GSK3) that the activity is inversely related of the glycogen content in the eggs in initial times, until to 3h, and from 48h until to the end of embryogenesis. Additionally, we cloned of one fragment of 600 bp related to the conserved region of GSK3 enzyme using RT-PCR from the 0h old eggs. Taken together these data suggest there is a high metabolism control during the Aedes aegypti embryogenesis.

Supported by PRONEX, FAPERJ, CNPq and CAPES.