Genome-Wide Analysis of Ty-Mediated Chromosomal
Rearrangements in Budding Yeast Cells Exposed to Ionizing Radiation.
Argueso, J.L.1,2;
Westmoreland, J.3; Mieczkowski, P.2; Gawel, G.2;
Resnick, M.3; Petes, T.2
1Campinas State University, Campinas, SP, Brazil;
2Duke University Medical
Center, Durham,
NC, USA;
3National Institute of Environmental Health Sciences, NIH, Research Triangle Park, NC, USA.
In this study we assessed the chromosomal consequences of
double-strand breaks (DSBs) in Saccharomyces cerevisiae under conditions
where there was no imposed selection for chromosomal changes. Despite very efficient
homologous DSB repair pathways, when we exposed yeast diploids to ionizing radiation
doses resulting in about 250 DSBs per cell more than half of the surviving
colonies exhibited altered karyotypes. To characterize the specific genome
rearrangements observed in radiation survivors, we employed microarray-based
Comparative Genomic Hybridization (CGH), which identifies regions of the genome
where an imbalance of gene dosage has resulted. Remarkably, the
radiation-induced chromosomal alterations appeared to be non-randomly
distributed, with the majority of the rearrangements identified in our data set
(86%) involving intra- or interchromosomal events between Ty insertions or solo
LTRs. Interestingly, specific Ty insertions were found as sites of
rearrangements much more frequently than expected. This was the case for a pair
Ty1 insertions on the right arm of chromosome V which represented 25% of all
Ty-mediated rearrangements. We also identified rearrangements between other
homeologous sequences which were not Ty-related (6%), such as a chromosomal
translocation between chromosomes IV and V mediated by the HXT15 and HXT13
loci. High levels of instability were also detected at genomic regions
containing the ribosomal DNA and CUP1 tandem repeats. To characterize
chromosomal aberrations which did not result in a gene dosage change we
isolated rearranged chromosomes from pulse-field gels and hybridized this DNA
to microarrays. This analysis revealed events which were missed by the CGH analysis, but
that were still the result of homologous recombination mediated by
Ty-associated sequences. Some of these rearrangements represented an unusual
class of recombination products in which two DNA ends (presumably formed by a
single DSB) engaged in recombination repair with distinct donor sequences. Taken
together, these observations indicate that homologous recombination was the
predominant pathway involved in the creation of chromosomal rearrangements, and
illustrate the importance that repetitive DNA sequences (i.e. Ty
insertions) have in genome dynamics. We suggest that the Ty-mediated
rearrangements are due to recombination between homologous elements competing
with recombinational repair between sister chromatids or homologous
chromosomes.
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