Fungi belonging to the genus Botryosphaeria are well-recognized as plant pathogens. They, along with other fungi, mainly basidiomycetes, produce exopolysaccharides (EPS); biopolymers having interesting biological activities (anti-tumor, anti-inflammatory), and commercial applications in foods, cosmetics and pharmaceutical products. However, for the applications of these macromolecules, it is first necessary to understand their chemical structures. An EPS (botryosphaeran) from Botryosphaeria rhodina (isolate MAMB-5) was recently characterized as a (1® 3;1® 6)-b -D-glucan, and studies showed that its production was stimulated using sucrose as carbon source. Therefore, the goal of this study was the production, purification and structural characterization of EPS produced by isolates of Botryosphaeria spp. from orange, mango, pinha and graviola fruits when grown on commercial sucrose as carbon source. The production of microbial polysaccharides was realized as described by Barbosa et al., 2003. Total sugars, reduced sugars and protein were determined, respectively, by the phenol-sulfuric acid (Dubois et al., 1956), cuproarsenate (Nelson, 1945) and protein-dye (Bradford, 1976) methods. Samples of EPS produced by the Botryosphaerial isolates were applied to a column of Sepharose CL-4B, and upon elution each showed a single peak indicating the material was homogeneous. Total acid hydrolysis of each EPS (TFA  5.0 M, 16 h, 100 ^oC) yielded only glucose as detected by high performance anionic exchange chromatography. FT-IR spectra showed one band at 891 cm^-1 indicating that all glucosidic linkages were of the b-configuration. These results indicated that the EPS from each Botryosphaerial isolate were b-glucans. Initial results of the analysis to determine structural conformation of the EPS from the Botryosphaerial isolates based upon the formation of complexes between EPS and the dye, Congo Red (Ogawa et al., 1972), in the presence of increased concentrations of NaOH (0.05 to 0.5 M) showed that the molecules probably have triple-helix conformation, and this aspect is important for biological activity.