Cássia multijuga (Senna multijuga) is a Leguminose tree, native from acid ground, used to ormament arborization, detached for your yellow flowers. Some of this species have been used in popular medicine for your anti-hepatotoxic and antimicrobian activity. The describe extraction and characterization of this polysacchacarides can be shown some biological activities. The polysaccharides were obtained by hot aqueous extraction, decoction and the residual material extracted successively with different concentration of aq. KOH at 80ºC. The polysaccharides were obtained by precipitation with excess EtOH. Thus were dialyzed and then submitted to freeze-thawing, giving a water-insoluble and soluble polysaccharides. The resultant fractions were analyzed by GC-MS of their derived alditol acetates, obtained on successive hydrolysis, NaBH₄ reduction and acetylation. The monosaccharides components of the fraction 1% KOH are rha:ara:xyl:gal:glc:uronic acid (0,3:3,7:61,8:6,8:1,9:25,6 molar ratio) and the fraction 2% KOH had ara:xyl:glc:uronic acid (1,3:87,2:2,4:9,2 molar ratio). After homogeneity analyze with HPSEC-MALLS, both of the fractions were submitted to a treatment with a-amylase, and then, the percentual of glc decrease. The 1% KOH fraction was submitted to a closed dialyze with constant agitation in a 16 kDa membrane. The resulting fraction has been submitted to a homogeneity analyze again, and purification with membranes with different sizes of pores. After that, the ¹³C-NMR analyzes will be done to elucidate the molecular structure of this fractions.

Supported by UNIOESTE- Cascavel and PRONEX- Carboidratos .