In according to the WHO, the tuberculosis kills 5000 people every day, if does not controlled, are esteemed 35 millions of new cases in next 20 years. The high susceptibility of human immunodeficiency virus infected persons to the disease and the proliferation of multidrug-resistant (MDR) strains have created a worldwide interest in expanding current programs in tuberculosis research. The Purine Nucleoside Phosphorylase is a potential target for new drug anti-tuberculosis, whereas is responsible for de novo synthesis of purine ribonucleotides. The specific inhibition of M. tuberculosis PNP could potentially lead to the latent state of M. tuberculosis. The objective of this work is to solve the structure from MtPNP complexed with adenine, and to analyze their interactions with the ligand. MtPNP was crystallized using the experimental conditions described elsewhere, and the ligand (adenine) was added by soaking. The X-ray diffraction data were collected on a CCD detector using synchrotron radiation source (Laboratório Nacional de Luz Síncrotron, LNLS, Campinas, Brazil). It was used the MOSFLM program to processing, and the data were scaled through the program SCALA, presenting orthorhombic spatial group P2₁2₁2 (a=118,96Å, b=134,65 Å, c=44,42 Å). The crystal was determined by molecular replacement methods using the program AMoRe. The final model has R_free and R_factor of 23.87% and 17.51% respectively, and maximum resolution of 1.86Å. It was observed a large electronic density in the center of the trimer, indicating the presence of adenine in this position. This fact has been the aim of our attention in the structure analysis.