XXXV Reunião Anual da SBBqResumoID:8020


Crystallization and preliminary X-ray crystallographic analysis of the complexes: Shikimate Kinase:ADP:shikimate and Shikimate Kinase:ADP:Mg2+ from Mycobacterium tuberculosis
Faim, LM1; Dias, MVB1; Vasconcelos, IB2; Oliveira, JS2; Basso, LA2; Santos, DS2; Azevedo Jr.,WF1,2

1 - IBILCE - UNESP - São José do Rio Preto SP;
2
- PUC - Porto Alegre - RS;


The seven step shikimate pathway links the metabolism of carbohydrates to the biosynthesis of aromatic amino acids and many aromatic secondary metabolites. It provides excellent potential targets for antimicrobial and agents because it is absent in animals but is essential in bacteria. Shikimate kinase is the fifth enzyme in this pathway and it catalyses the phosphorylation of the 3-hydroxyl group of shikimate using ATP as a co-substrate. In this work, we present crystallization and preliminary X-ray crystallographic analysis of shikimate kinase from Mycobacterium tuberculosis (MtSK) in two complexes: MtSK:ADP:shikimate and MtSK:ADP:Mg2+. For crystallization of these two complexes were used the hanging drop vapor diffusion and sparse matrix methods. X-ray diffraction data sets were collect at wavelength of 1.427Ǻ using the Synchrotron Radiation Source. The data were processed using the program Mosflm and Scala. The complex MtSK:ADP:shikimate was crystallized in Tris–HCl, 17% of PEG 1500 and 0.5–0.7M of LiCl, while the complex MtSK:ADP:Mg2+ was crystallized in Tris–HCl, 25% of PEG 3350 and 0,1M of MgCl2. X-ray diffraction data were processed to resolution of 1.93Ǻ and  2.8Ǻ to MtSK:ADP:shikimate and to MtSK:ADP:Mg2+, respectively. The crystals of MtSK:ADP:shikimate are trigonal and belong to P3221 space group with unit cell dimensions of a=b=63.3 and c=91.6Å and present one molecule per asymmetric unit. The crystals of MtSK:ADP:Mg2+ are orthorhombic and belong to P212121 space group with unit cell dimensions of a=60.62, b=62.20 and c=170.63Å and present four molecules per asymmetric unit. The data sets present here posses an Rsym below of 12.1% and completeness above of 96.9%. With these results we intend to determine the crystallographic structure of MtSK for these two complexes and to verify the possible conformational changes caused for Mg2+ and for shikimate molecule. Furthermore these data can be until in the development new drugs against infectious disease. Fapesp (processes 05/50446-9, 03/12472-2 and 01/07532-0).