The goal of this study was to prepare and characterize lectinized-magnetoliposomes (LML) and to evaluate the interaction of lectin and lipid vesicles. In this framework, interactions that may occur between wheat germ agglutinin lectin (WGA) and phospholipids in mixed monolayers were investigated. WGA (29mM) and phospholipids (1mM dipalmitoylphosphatidylcholine-DPPC, dioleoylphosphatidylcholine-DOPC, dibehenoylphosphatidylcholine-DBPC, and dipalmitoylphosphatidyletanolamine-DPPE) were analyzed using Langmuir technique, fluorescence and impedance spectroscopy. Initially, magnetic nanoparticles (68 ±18 nm) were prepared by co-precipitation of 0.12M FeCl₃ and 0.05M FeSO₄. Then LML was obtained by the thin film technique, followed by redispersion with colloidal magnetite and WGA solution. Multilamellar liposomes were submitted to sonication to obtain unilamellar vesicles. p–A isotherms showed that WGA had a mean molecular area of 3,800 Ų and support the existence of its interaction with phospholipids, especially in DOPC mixed films (1,320 Ų/molecule). LML was obtained with 169 ± 72nm mean diameter of vesicles. Fluorescence spectroscopy showed that WGA has low interaction with DPPE-LML. Conversely, WGA has a great interaction with DOPC-LML. The quenching in the WGA fluorescence of DOPC-LML spectra also indicated an interaction between the lectin and the phospholipid bilayer. Low values of the impedance real part of magnetite (Z_re=1.98 kW) were detected from dielectric relaxation diagrams, while for WGA and unloaded liposomes Z_re is about 10⁵ W. LML presented Z_re values similar to unloaded liposomes, preserving the dielectric properties of pure lipids. This feature indicates that magnetite was entrapped into the lipid vesicles. Additionally, the analysis of the hysteresis cycle of magnetite showed that such particles were paramagnetic. From these results we can state that LML were obtained, which has potential application in magnetic resonance imaging and drug delivery systems for cancer therapy.