Free radicals are continuously formed by normal process of metabolism and transition metal ions can catalyse their production through Fenton reaction. The excess of free radicals is implicated in the etiology of several diseases and aging. The interactions between a new flavonoid (floranol) with Fe(III) ions were investigated by UV-Vis spectroscopy and electrospray ionisation mass spectrometry (ESI-MS). The acidity constants of the ligand as well as the stability constants of the Fe(III) complexes were calculated. Floranol has two ionizable protons in the pH range studied. Floranol exhibits two absorption bands at 340 (band I) and at 297 nm (band II) related to the p-p transitions of chromophores B and A, respectively. Between pH 2.0 and 8.0, band II decreases in intensity and band I increases, with an isosbestic point at 313 nm. Between pH 8.5 and 12.0 the intensity of band I decreases and shifts to 327 nm. The pKa values of 6.58 and 11.97 were determined. Addition of Fe(III) decreases the intensity of band II between pH 2.1 and 5.0 and, from pH 5.6 to 6.9, increases the intensity of band I. Up to pH to 11.5, a shoulder centered at 398 nm appears, which was assigned to a charge transfer transition from the pp phenolate orbital of the ligand to the partially filled dp* orbital of Fe(III). The best fit between experimental and calculated spectra was obtained assuming the formation of three complexes: [FeL₃] logb = 44.72 ± 0.09, [FeL₂] logb  = 35.32 ± 0.08, and [FeL] logb =19.51 ± 0.04. The ESI-MS studies provided a direct evidence for the formation of the complexes observed in the spectrophotometric study. The FeL₃, FeL₂, and FeL complexes were detected at 1213, 827, and 441 m/z, respectively. Furthermore, the data obtained by Collision Induced Dissociation shows that complexes between floranol and iron are very stable. These findings suggest that floranol may play an important role in metal-overload diseases such hemochromatosis (iron overload) and atherosclerotic diseases by inactivation of iron on generating reactive species.