The study about biodegradable and biocompatible polysaccharide films on solid surfaces has technological interest due to its potential utilization as biomaterials and controlled liberation of drugs systems. Besides this, proteases have been pointed as an interesting drug, acting along wound healing process (inflammatory phase and collagen remodelling). The aim of this work was to immobilize trypsin by entrapment onto polysaccharide films from Anacardium occidentale gum (POLICAJU) and to evaluate the following parameters: pH, temperature and stabilities, which affect enzyme activity, looking for its application as cutaneous dressings. The films were obtained after solubilization of POLICAJU [10% (w/v)  in 4% acetic acid  (v/v)], under agitation for 16 h at 25 ºC. Then, pH was adjusted to 5.0 using 1M NaOH. Afterwards, powdered trypsin (1000 mg/ml) was added to the system and shaked for 1 h. Aliquots (200 ml) were placed on 1.0 cm² of cellulosic support and the films were dried at 50 + 5 °C. The optimum pH for free and immobilized enzyme (DEI) was pH 10.2 e pH 8.6, respectively. The optimum trypsin temperature was 55 ^oC for both situations, however was observed that DEI showed a wide thermal stability range when compared to free enzyme. The DEI retained 100% of its activity after 90 min at pH 5.0 and 6.2, as well as after 60 min at 25°C, 35°C e 45°C of temperature. When DEI was tested at room stock stability, it was observed that it retained 80% of its initial activity when stored for 28 days at room temperature. The results show that the POLICAJU presents a good potential for aplication as immobilization matrix of proteases and possible utilization for treatment of cutaneous wounds.