Production of xylanase d proteases by Trichoderma aureovirideae used sugar cane as substrate
Carneiro da Cunha, M.N. 1,2; Albertini, A.V.P. 2,6; Viana, D.A. 2,6; Moreira, K.A. 2,7; Soares, M.T.C.V. 2,4;Souza-Mota, C.M. 5;Lima –Filho, J.L.2,3 and Porto, A. L.F.2,4
1Bolsista PIBIC-CNPq/ UFRPE;2Laboratório de Imunopatologia Keizo Asami-LIKA/UFPE;3Departamento de Bioquímica/UFPE;4Departamento de Morfologia e Fisiologia Animal/UFRPE;5Departamento de Micologia/UFPE;6Programa de Pós-Graduação em Ciências Biológicas/UFPE;7Unidade Acadêmica de Garanhuns/UFRPE.
Xylanases catalyse the hidrolysis of xylans and are produced mainly by microorganisms. Among microbial sources, filamentous fungi are specially interesting as they secrete enzymes into the medium, may be industrially produced in submerged liquid or on solid state culture. Xylanases are applicated in the animal feed production, bread-making, wine and fruit and vegetables industries, pharmaceutical and chemical area. The aim of this work was xylanase production by Trichoderma aureovirideae used sugar cane as only substrate. The microrganism was isolated from the root sugar cane and indentified by Departament of Micology/UFPE. The T. aureovirideae growth in solid state culture in Erlenmeyers (125 mL) containing 10 g sugar cane in orbital shaker (180 rpm) at 30˚C for 120 hours. The enzymes were obtained from supernatant after extraction with saline solution (150mM) and centrifugation (5500 xg) for 15 minutes. Analytical determinations were carried out for xylanase and protease activities and total protein. The results showed 2062 U/mL of maximum xylanase activity with 120 hours of the cultive, these results were higher than that reported in the literature. Protease production was very low (0.33 U/mL ). However, the co-production of proteolytic activity may be a serios problem for the maintenance of xylanases stability. Fortunately, low levels proteolytic activity produced during the fermentation of sugar cane by T. aureovirideae. These results may be explained by the chemical composition of material, which contained low protein in their composition. The fungi demonstrated be a good producer of xylanase in solid state culture, it could be used in several biotechnological process of industrial interest.
Suppoted by PROMATA-FACEPE, FINEP and CNPq
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