Dengue infection affects millions of people worldwide and represents a serious issue of public health. Its clinical manifestations may include a series of hemostatic alterations, and several evidences indicate that, at least for the more severe cases, liver function is heavily compromised. The liver secretes cytokines and the acute phase proteins involved in the inflammatory response. Thus, studies about the effects of dengue virus replication on the physiology of liver cells will certainly contribute to the understanding of dengue disease. In order to identify differentially secreted proteins from HepG2 cells (an human hepatoma cell lineage), infected or not with dengue virus, a microarray approach was used. We have analyzed expressed RNA from HepG2 cells infected or not with Dengue-2 virus after 48 hours of infection. After RNA isolation, cDNA was  synthezised and labeled with Cy3-dCTP or Cy5-dCTP. The labeled cDNA were hybridized in a microarray library from human genes that contain more than 10,000 genes of known function or with homology to known genes. Differential expression of genes was analyzed with genArise software where we calculated the Z-score and identified  genes which expression was changed.  Afterwards genes were classified using kegg metabolic pathway. Up regulated genes include: basal transcription factors, MAPK signaling, citokine-citokine receptor interaction, neuractive ligand-receptor interaction, Jak-STAT singaling, complement/coagulation cascades  and regulation of active cytoskelenton. Down-regulated genes include: calcium signaling, apoptosis, Wnt signaling pathway, ribosome assembly, protein synthesis, complement/coagulation cascade and insulin pathway. The significane of altering these genes in the physiology of the cell is being examined. Supported by ICGEB-OPS_RELAB, CNPq, FAPERJ