Several evidence have found a close association between oxidative stress and pathogenesis of osteoporosis in diabetes mellitus. Since Reactive Oxygen Species (ROS) are increased in diabetes mellitus and play an important role in the pathogenesis of the typical long-term diabetic complication like osteopenia, we examined the effect of mineral and Vitamin E supplementation on bone metabolism of diabetic male rats (180-220 g) over 60 days after onset of experimental diabetes (i.p. streptozotocin 40 mg/Kg body weight) with glycemia ≥ 250 mg/dL . Three groups of 10 rats were fed: Control (C), diabetics (D) and diabetic supplemented (DS) with calcium and phosphorus (2.5x) and vitamin E (20x). The animals were sacrificed at days 5, 30 and 60 and calcium, phosphorus, magnesium, creatinine, albumin, glucose, alkaline phosphatase and acid phosphatase were measured using routine methods. Statistical analyses (analysis of variance – ANOVA) were performed with Statisitc Program, version 6.0 (Statsoft,Tulsa, OK, USA). No difference were observed for serum analysis except for phosphatases. Alkaline phosphatase showed similar activities on days 5 and 30 for all groups (250 ± 4.7U/L) and on day 60 an increase of twofold for D (170± 7.0U/L) and DS (178± 7.0U/L) when compared to controls (79± 4.0U/L). Acid phosphatase showed a tendency of increase for diabetics (32± 1.0U/L) and decrease for DS (29± 0.8U/L) when compared to controls (23± 0.7U/L), over 60 days. The supplementation with Vitamin E, a well recognized antioxidant, in addition of calcium and phosphorus, besides the fact that histomorphometric analyses showed a significant reduction of bone loss (not shown), suggested a protective role of the supplementation in bone turnover and in diabetic osteopenia.