Candida albicans is an fungal opportunistic specie that can be found in skin, oral cavity, vaginal canal and gastrointestinal tract which may act as pathogen, establishing infections that can be dangerous when the immune responses are greatly compromised. Species of Sapindus saponaria, known as "sabão de soldado", has their fruits used by population against ulcers, skin lesions and inflammations. The aim of this work was study the antifungal capacity of ripe (FM) and green (FV) fruits metanolic extracts, metanolic (Cmet) and aqueous (Caq) callus culture extracts. The evaluation was performed by broth microdilution antifungal susceptibility tests according NCCLS M27A against the species Candida albicans ATCC 90028 (Ca) and two isolates obtained from vaginal tract and oral cavity (Cv; Co). The preliminary results showed that the Minimal Inhibitory Concentration (MIC) to Ca was 1250 µg/mL to FM and FV extracts, while to strain isolated from vaginal tract, MIC values were 625 µg/mL. The FM and FV extracts inhibit the growth of oral strain with MICs of 1250 µg/mL and 625 µg/mL, respectively. The metanolic and aqueous extracts obtained from callus culture were tested only to C. albicans and showed MIC values of 625 and 312,5 µg/mL, respectively. The preliminary results support evidences to the use of the broth microdilution test as a powerful methodology to analyze the antifungal capacity of plant extracts since there is no standard reference method recommended to plant extracts. It can be concluded that callus culture extracts of S. saponaria presents antifungal activity due to the low levels of MIC obtained suggesting that these extracts may be an important biotechnological tool directed to the discovery of new therapeutic agents.